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©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Sep 26, 2025; 17(9): 106282
Published online Sep 26, 2025. doi: 10.4252/wjsc.v17.i9.106282
Published online Sep 26, 2025. doi: 10.4252/wjsc.v17.i9.106282
Bone marrow mesenchymal stem cell-derived exosomal lactate dehydrogenase A promotes tendon-bone healing via histone lactylation-mediated cartilage regeneration
Ting Zhang, Kai-Feng Gan, Department of Orthopedics, The Affiliated Lihuili Hospital of Ningbo University, Ningbo 315211, Zhejiang Province, China
Qing Huang, Department of Gynaecology, The Affiliated Lihuili Hospital of Ningbo University, Ningbo 315211, Zhejiang Province, China
Author contributions: Zhang T designed the study; Huang Q conduced the experiments; Gan KF wrote the manuscript.
Institutional review board statement: The study was approved by the Affiliated Lihuili Hospital of Ningbo University Medical Ethics Committee (Approval No. LHL112532).
Institutional animal care and use committee statement: The study was approved by the Affiliated Lihuili Hospital of Ningbo University Medical Ethics Committee for Laboratory Animals (Approval No. LHLS-1059), and complied with the Guidelines for the Management and Use of Laboratory Animals.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: The datasets used and analysed during the current study are available from the corresponding author on reasonable request.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Kai-Feng Gan, Department of Orthopedics, The Affiliated Lihuili Hospital of Ningbo University, No. 57 Xingning Road, Ningbo 315211, Zhejiang Province, China. gankaifeng@163.com
Received: February 26, 2025
Revised: March 27, 2025
Accepted: September 5, 2025
Published online: September 26, 2025
Processing time: 209 Days and 20.8 Hours
Revised: March 27, 2025
Accepted: September 5, 2025
Published online: September 26, 2025
Processing time: 209 Days and 20.8 Hours
Core Tip
Core Tip: This study reveals that lactate dehydrogenase A (LDHA) in exosomes from bone marrow mesenchymal stem cells significantly enhances tendon-bone healing after anterior cruciate ligament reconstruction. LDHA promotes chondrocyte stem cell proliferation and differentiation by increasing lactate secretion and upregulating bone morphogenetic protein 7 via histone H3K18 lactylation. High LDHA expression boosts cartilage and fibrocartilage regeneration, accelerates new bone formation, and reverses bone morphogenetic protein 7 knockdown effects. These findings highlight LDHA-rich exosomes from bone marrow mesenchymal stem cells as a promising therapeutic strategy for improving anterior cruciate ligament reconstruction outcomes.