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©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Oncol. Dec 24, 2025; 16(12): 112161
Published online Dec 24, 2025. doi: 10.5306/wjco.v16.i12.112161
Published online Dec 24, 2025. doi: 10.5306/wjco.v16.i12.112161
Expression patterns and clinical implications of chaperonin subunit 3 mRNA and protein in laryngeal squamous cell carcinoma
Bin-Yu Mo, Shi-Hua Yin, Department of Otolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Guangxi Medical University, Nanning 530007, Guangxi Zhuang Autonomous Region, China
Bin-Yu Mo, Zi-Li Qin, Fang-Yun Tian, Department of Otolaryngology Head and Neck Surgery, Liuzhou People’s Hospital Affiliated to Guangxi Medical University, Liuzhou 545000, Guangxi Zhuang Autonomous Region, China
Jia-Ying Wen, Department of Radiotherapy, The Second Affiliated Hospital of Guangxi Medical University, Nanning 530007, Guangxi Zhuang Autonomous Region, China
Guo-Qiang Chen, Jing-Wen Ling, Han He, Qi Li, Bin Li, Jian-Di Li, Zong-Yu Li, Gang Chen, Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
Jing-Wen Ling, Department of Medical Information Engineering, School of Information and Management, Nanning 530199, Guangxi Zhuang Autonomous Region, China
Rong-Quan He, Department of Medical Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
Di-Yuan Qin, Department of Computer Science and Technology, School of Computer and Electronic Information, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China
Chao-Hua Mo, Department of Pathology, Xiaolan People’s Hospital of Zhongshan, Xiaolan People’s Hospital of Zhongshan, Zhongshan 528415, Guangdong Province, China
Chao-Hua Mo, Department of Pathology, Foshan Hospital of Traditional Chinese Medicine, Zhongshan 528000, Guangdong Province, China
Chang Chen, Department of Pathology, Wuzhou Red Cross Hospital, Wuzhou 543000, Guangxi Zhuang Autonomous Region, China
Li Yang, Department of Pathology, The Second Affiliated Hospital of Guangxi Medical University, Nanning 530007, Guangxi Zhuang Autonomous Region, China
Co-first authors: Bin-Yu Mo and Jia-Ying Wen.
Co-corresponding authors: Shi-Hua Yin and Li Yang.
Author contributions: Mo BY and Wen JY contributed equally to this article, they are the co-first authors of this manuscript; Mo BY, Wen JY, He RQ, Chen G, Yin SH, and Yang L designed the research project, supervised various experiments and statistical analyses, and revised the manuscript; Chen GQ, Ling JW, He H, Li JD, Li ZY, and Chen C performed large-scale data screening, and re-analysis of gene microarray and RNA-sequencing data; Mo CH, Qin ZL, Tian FY, Li Q, and Li B conducted the clinical pathology study, including immunohistochemical experiments and assessment; Qin DY performed data visualization; Yin SH and Yang L contributed equally to this article, they are the co-corresponding authors of this manuscript; and all authors thoroughly reviewed and endorsed the final manuscript.
Supported by the National Natural Science Foundation of China, No. 82160213 and No. U22A2022; and the Guangxi Natural Science Foundation, No. 2023GXNSFAA026029, No. 2024GXNSFBA010059, and No. 2024GXNSFAA010079.
Institutional review board statement: This study was approved by the Medical Ethics Committee of Wuzhou Red Cross Hospital, approval No. LL2024-116; Foshan Hospital of Traditional Chinese Medicine, No. KY[2022]193; and Guilin Fanpu Biotechnology Company Limited, approval No. Fanpu[2018]23.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: All data included in this study are available upon request by contact with the corresponding author.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Shi-Hua Yin, Professor, Department of Otolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Guangxi Medical University, No. 166 East Daxue Road, Xixiangtang District, Nanning 530007, Guangxi Zhuang Autonomous Region, China. shihuayin@gxmu.edu.cn
Received: July 21, 2025
Revised: September 13, 2025
Accepted: November 20, 2025
Published online: December 24, 2025
Processing time: 157 Days and 2.8 Hours
Revised: September 13, 2025
Accepted: November 20, 2025
Published online: December 24, 2025
Processing time: 157 Days and 2.8 Hours
Core Tip
Core Tip: In this study, single-cell RNA-seq and functional clustered regularly interspaced short palindromic repeats screening demonstrated the overexpression of chaperonin-containing TCP1 subunit 3 in laryngeal squamous cell carcinoma, thereby showing its role in promoting cell viability. Gene set enrichment analysis indicated that chaperonin-containing TCP1 subunit 3 is involved in extracellular matrix receptor interaction and cell cycle pathways, highlighting its potential as a biomarker and therapeutic target for laryngeal squamous cell carcinoma.