Abnormal expression and potential clinical value of oncogenic Krüppel-like factor-5 in lung squamous cell carcinoma
Yang Shi, Wen-Li Sai, Jin-Liang Chen, Li-Wei Qiu, Min Yao, Jun Zhao, Deng-Fu Yao
Yang Shi, Wen-Li Sai, Li-Wei Qiu, Deng-Fu Yao, Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China
Yang Shi, Department of Thoracic Surgery, Affiliated Yancheng Hospital of Nantong University, Yancheng 226014, Jiangsu Province, China
Jin-Liang Chen, Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China
Min Yao, Department of Immunology, Medical School of Nantong University, Nantong 226001, Jiangsu Province, China
Jun Zhao, Department of Thoracic Surgery, The First Affiliated Hospital of Suzhou University, Suzhou 215008, Jiangsu Province, China
Co-first authors: Yang Shi and Wen-Li Sai.
Co-corresponding authors: Jun Zhao and Deng-Fu Yao.
Author contributions: Shi Y, Sai WL, and Chen JL conceptualized and designed the research; Qiu LW and Yao M screened patients and acquired clinical data; Sai WL and Chen JL collected blood specimen and performed laboratory analysis; Shi Y and Chen JL collected blood specimen and performed laboratory analysis; Yao DF and Zhao J supervised the study and revised the manuscript; Shi Y, Yao M, Sai WL, Chen JL and Qiu LW constructed the layout of the figures. All the authors read and approved the final manuscript. Shi Y proposed, designed and conducted serum KLF-5 analysis, performed data analysis and prepared the first draft of the manuscript. Chen JL was responsible for patient screening, enrollment, collection of clinical data and blood specimens. Both authors have made crucial and indispensable contributions towards the completion of the project and thus qualified as the co-first authors of the paper. Both Yao DF and Zhao J have played important and indispensable roles in the experimental design, data interpretation and manuscript preparation as the co-corresponding authors. Chen JL applied for and obtained the funds for this research project. Yao DF conceptualized, designed, and supervised the whole process of the project. He searched the literature, revised and submitted the early version of the manuscript with the focus on the association between LUSC and KLF5. Yao DF was instrumental and responsible for data re-analysis and re-interpretation, figure plotting, comprehensive literature search, preparation and submission of the current version of the manuscript with a new focus on KLF5 as the predictors of LUSC and on potential underlying mechanisms. This collaboration between Zhao J and Yao DF is crucial for the publication of this manuscript and other manuscripts still in preparation.
Supported by Jiangsu Commission of Health of China, No. M2020096.
Institutional review board statement: The research was carried out in accordance with the most recent version of the Helsinki Declaration and was approved by the Independent Interdisciplinary Ethics Committee for the Affiliated Hospital of Nantong University (No. 2020-L151). Each participant provided informed consent for their involvement in the study and for the publication of anonymized results, after the procedures had been thoroughly explained.
Institutional animal care and use committee statement: Ethical approval for the was provided by the Animal Care and Use Committee of Nantong University (No. S20220221-009), China.
Conflict-of-interest statement: All authors declare that there are no competing interests.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
https://creativecommons.org/Licenses/by-nc/4.0/ Corresponding author: Deng-Fu Yao, MD, PhD, Postdoc, Professor, Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, No. 20 West Temple Road, Nantong 226001, Jiangsu Province, China.
yaodf@ahnmc.com
Received: June 26, 2025
Revised: August 4, 2025
Accepted: September 25, 2025
Published online: October 24, 2025
Processing time: 121 Days and 3.6 Hours
