Mesenchymal stem-derived exosomes enhance therapeutic benefits of exercise in isoproterenol-induced myocardial ischemia: Targeting ERK and Akt/mTOR signaling

doi:10.4252/wjsc.v17.i10.109862

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Oct 26, 2025; 17(10): 109862
Published online Oct 26, 2025. doi: 10.4252/wjsc.v17.i10.109862

Table 1 Primer’s sequence

Gene		Primer sequence (5’-3’)
Akt	Forward	CACTTTCGGCAAGGTGATCC
Akt	Reverse	GTCCTTGGCCACGATGACTT
mTOR	Forward	AGCATCGGATGCTTAGGAGTGG
mTOR	Reverse	CAGCCAGTCATCTTTGGAGACC
MMP9	Forward	TATTTTTGT GTGGCGYCTGAGAA
MMP9	Reverse	GAGGTGGTTTAGCCGGTGAA
ERK	Forward	GAGCCCAGGGGAACTGCT
ERK	Reverse	TGGAAGCGGGCTGTCTC
SERCA2	Forward	CAGTTCATCCGCTACCTCATCTCC
SERCA2	Reverse	CGCAGTGGCAGGCAGACC
β-actin	Forward	AGGTCGGAGTCAACGGATTTGGT
β-actin	Reverse	CATGTGGGCCATAGGTCCACCAC

Akt: Protein kinase B; mTOR: Mammalian target of rapamycin; MMP9: Matrix metalloproteinase 9; ERK: Extracellular signal-regulated kinase; SERCA2: Sarcoplasmic endoplasmic reticulum calcium ATPase type 2.

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Citation: ShamsEldeen AM, AbdElalim MM, Mohamed NS, AbdelRahman MM, Kamar SS, AbdelKader DH, Elsharkawy SH, Rashed LA, Faisal SS, Osman WA, Selmy AM. Mesenchymal stem-derived exosomes enhance therapeutic benefits of exercise in isoproterenol-induced myocardial ischemia: Targeting ERK and Akt/mTOR signaling. World J Stem Cells 2025; 17(10): 109862
URL: https://www.wjgnet.com/1948-0210/full/v17/i10/109862.htm
DOI: https://dx.doi.org/10.4252/wjsc.v17.i10.109862