Published online Mar 26, 2024. doi: 10.4252/wjsc.v16.i3.287
Peer-review started: December 19, 2023
First decision: January 12, 2024
Revised: January 21, 2024
Accepted: February 28, 2024
Article in press: February 28, 2024
Published online: March 26, 2024
Processing time: 96 Days and 18.9 Hours
Stem cells can self-organise into microsized organ units, which can partially model tissue function and regeneration. Dental pulp organoids have been used to replicate the processes of tooth development and related diseases. However, the lack of vasculature limits the usefulness of dental pulp organo.
The survival of stem cell transplants should be promoted, thereby improving the repair ability of the cells.
Three-dimensional (3D) self-assembly of a novel vascularised dental pulp-like organoid in vitro by hypoxia and con
Human dental pulp stem cells were induced from endothelial cells (ECs) through exposure to a hypoxic environment and conditioned medium. The resulting cells were then mixed with ECs at specific ratios and conditioned in a 3D environment to produce Vorganoids. The biological characteristics of the Vorganoids were analysed, and the regulatory pathways associated with angiogenesis were studied.
Vorganoids are similar in morphology and function to dental pulp tissue. Single-cell RNA sequencing of dental pulp tissue and RNA sequencing of Vorganoids were performed to identify the involvement of the biomarkers forkhead box protein O1 (FOXO1) and fibroblast growth factor 2 (FGF2) in key regulatory pathways associated with Vorganoid angiogenesis.
In this study, we effectively established an in vitro model of prevascularized dental pulp organoids and used it to elucidate novel mechanisms of angiogenesis during dental regeneration. The biomarkers FOXO1 and FGF2 confirmed the angiogenesis-regulating role of angiopoietins.
This innovative study has effectively established an in vitro model of prevascularized dental pulp organoids and used it to elucidate new mechanisms of angiogenesis during regeneration, facilitating the development of clinical treatment strategies.