Jiang Y, Huang WJ, Zhou R. Advancing human adipose-derived mesenchymal stem cell-derived exosomes for androgenetic alopecia: Appraisal and methodological recommendations. World J Stem Cells 2025; 17(12): 113456 [DOI: 10.4252/wjsc.v17.i12.113456]
Corresponding Author of This Article
Rui Zhou, PhD, Associate Professor, Shaanxi Institute for Pediatric Diseases, Affiliated Children’s Hospital of Xi’an Jiaotong University, No. 69 West Juyuan Lane, Lianhu District, Xi’an 710003, Shaanxi Province, China. zhouhuaxizhu@126.com
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Dermatology
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Letter to the Editor
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This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Dec 26, 2025 (publication date) through Dec 31, 2025
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World Journal of Stem Cells
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1948-0210
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Jiang Y, Huang WJ, Zhou R. Advancing human adipose-derived mesenchymal stem cell-derived exosomes for androgenetic alopecia: Appraisal and methodological recommendations. World J Stem Cells 2025; 17(12): 113456 [DOI: 10.4252/wjsc.v17.i12.113456]
World J Stem Cells. Dec 26, 2025; 17(12): 113456 Published online Dec 26, 2025. doi: 10.4252/wjsc.v17.i12.113456
Advancing human adipose-derived mesenchymal stem cell-derived exosomes for androgenetic alopecia: Appraisal and methodological recommendations
Yu Jiang, Wen-Jun Huang, Rui Zhou
Yu Jiang, Department of Emergency Medicine, The Traditional Chinese Medicine Hospital of Longquanyi, Chengdu 610100, Sichuan Province, China
Wen-Jun Huang, Rui Zhou, Shaanxi Institute for Pediatric Diseases, Affiliated Children’s Hospital of Xi’an Jiaotong University, Xi’an 710003, Shaanxi Province, China
Author contributions: Jiang Y was responsible for drafting the manuscript; Huang WJ carried out proofreading and formatting checks; Zhou R contributed to the conceptualization, supervision, and proofreading of the manuscript. All authors have reviewed and approved the final version for publication.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Rui Zhou, PhD, Associate Professor, Shaanxi Institute for Pediatric Diseases, Affiliated Children’s Hospital of Xi’an Jiaotong University, No. 69 West Juyuan Lane, Lianhu District, Xi’an 710003, Shaanxi Province, China. zhouhuaxizhu@126.com
Received: September 3, 2025 Revised: September 30, 2025 Accepted: November 21, 2025 Published online: December 26, 2025 Processing time: 113 Days and 14.7 Hours
Abstract
Fu et al investigated human adipose-derived mesenchymal stem cell exosomes for androgenetic alopecia, identifying a stable set of 232 proteins and proposing the CDC42-Wnt/β-catenin- glycogen synthase kinase 3β signaling axis. These findings support the potential for clinical translation. To further strengthen clinical relevance, we recommend: (1) More comprehensive exosome characterization, including key marker analysis; (2) Reporting both particle count and protein concentration, and establishing dose-response relationships; (3) Direct validation of CDC42’s role through knockout or overexpression experiments; (4) Multi-level evaluation of Wnt/β-catenin signaling; (5) Enhanced biochemical validation in animal models; (6) Quantitative analysis of microneedle delivery parameters and retention; and (7) Early development of a streamlined quality control and safety framework. These methodological advancements will help exosome-based therapies better align with emerging regulatory standards and clinical guidelines. By addressing these aspects, future research can facilitate the safe, effective, and reproducible application of exosome-based treatments in clinical practice.
Core Tip: Key challenges for translating human adipose-derived mesenchymal stem cell-derived exosomes in androgenetic alopecia include: Incomplete exosome marker information; lack of standardized reporting for particle counts and protein content; absence of direct CDC42 function validation; limited depth in Wnt/β-catenin pathway analysis; insufficient biochemical data in animal models; undefined microneedle delivery parameters; limited safety profiling; and lack of integrated quality control and potency release criteria.
