Stem cell collection from peripheral blood of multiple myeloma patients

Table 1 Clinical protocol for stem cell collection from rural multiple myeloma patients in one trip

	Friday	Saturday	Sunday	Monday	Tuesday	Wednesday	Thursday	Friday
Event	Mobilization day 1	Mobilization day 2	Mobilization day 3	Mobilization day 4	Collection day 1	Collection day 2	Collection day 3	Collection day 4
AM	G-CSF	G-CSF	G-CSF	G-CSF	G-CSF	G-CSF	G-CSF	G-CSF
PM				Plerixafor	Plerixafor	Plerixafor	Plerixafor
			Arrival in town		Possible departure for home	Possible departure for home	Possible departure for home	Departure for home

G-CSF: Granulocyte-colony stimulating factor.

Table 2 Patient 1 treatment courses and response

Patient 1 (59 M)	Monday	Tuesday	Wednesday	Thursday	Two weeks following collection
Event	Mobilization Day 4 (Day 4 GSF; pre-collection)	Collection Day 1 (Day 5 G-CSF)	Collection Day 2	Collection Day 3 (collection complete)	HDC (Melphalan 200 mg/m2)
pCD34 (cells/μL)	2.10	22.03	32.20	11.40
WBC (cells/μL)	14.60 × 103	28.70 × 103	42.10 × 103	39.50 × 103
Product cell count (CD34 cells/kg)		3.45 × 106	3.94 × 106	1.42 × 106
Total yield (CD34 cells/kg)				8.81 × 106
Infusion (CD34 cells/kg)					4.16 × 106

G-CSF: Granulocyte-colony stimulating factor; WBC: White blood cell; HDC: High-dose chemotherapy.

Table 3 Patient 2 treatment courses and response

Patient 2 (62 F)	Monday	Tuesday	Wednesday	Thursday	Friday	Two weeks following collection
Event	Mobilization Day 4 GSF (pre-collection)	Collection Day 1 (Day 5 G-CSF)	Collection Day 2	Collection Day 3	Collection Day 4 (Collection complete)	HDC (Melphalan 200 mg/m2)
pCD34 (cells/μL)	1.30	22.03	15.70	14.00	14.10
WBC (cells/μL)	15.07 × 103	22.41 × 103	19.55 × 103	23.14 × 103	25.53 × 103
Product cell count (CD34 cells/kg)		1.79 × 106	2.67 × 106	2.76 × 106	2.40 × 106
Total yield (CD34 cells/kg)					9.62 × 106
Infusion (CD34 cells/kg)						3.12 × 106 CD34 cells/kg

G-CSF: Granulocyte-colony stimulating factor; WBC: White blood cell; HDC: High-dose chemotherapy.

Table 4 Patient 3 treatment courses and response

Patient 3 (67 M)	Monday	Tuesday	Wednesday	Thursday
Event	Mobilization Day 4 GSF (pre-collection)	Collection Day 1 (Day 5 G-CSF)	Collection Day 2	Collection Day 3 (collection complete)
pCD34 (cells/μL)	2.30	15.50	21.20	30.20
WBC (cells/μL)	15.20 × 103	20.70 × 103	28.70 × 103	29.60 × 103
Product cell count (CD34 cells/kg)		2.23 × 106	3.39 × 106	4.96 × 106
Total yield (CD34 cells/kg)				10.58 × 106
Infusion (CD34 cells/kg)

G-CSF: Granulocyte-colony stimulating factor; WBC: White blood cell.

Table 5 Aggregate patient collection summary

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Patient	Age	Sex	Pre-collection pCD4 count (cells/μL)	Collection day 1 pCD34 count (cells/μL)	Collection day 2 pCD34 count (cells/μL)	Collection day 3 pCD34 count (cells/μL)	Collection day 4 pCD34 count (cells/μL)	Total apheresis yield (CD34+ cells/kg)	Infusion (CD34+ cells/kg)
Patient 1	59	Male	2.10	22.03	32.20	11.40		8.81 × 106	4.16 × 106
Patient 2	62	Female	1.30	22.03	15.70	14.00	14.10	9.62 × 106	3.12 × 106
Patient 3	67	Male	2.30	15.50	21.20	30.20		10.58 × 106