Transcription factor 3 enhances hepatocellular carcinoma metastasis by upregulating matrix metalloproteinase-11

Table 1 Correlation between transcription factor 3 expression and Clinicopathological factors of patients with hepatocellular carcinoma

Variables	TCF3 expression of low (n = 41)	TCF3 expression of high (n = 35)	P value
Age (years)
< 60	19	15	0.761
≥ 60	22	20
Gender
Male	23	20	0.927
Female	18	15
Hepatitis B virus infection
Negative	12	6	0.215
Positive	29	29
Cirrhosis
Absent	13	10	0.767
Present	28	25
Alpha-fetoprotein
Elevatory	26	26	0.310
Normal	15	9
Differentiation
Well	16	8	0.0326
Moderate	20	14
Poor	5	13
Tumor size
≤ 5 cm	25	16	0.183
> 5 cm	16	19
Number of tumors
Single	33	22	0.087
Multiple	8	13
Lymph node metastasis
Absent	29	11
Present	12	24	0.001
Microvascular invasion
Absent	31	18	0.028
Present	10	17
American Joint Committee on Cancer stage
I	15	3	0.0107
II	6	11
III	20	21

TCF3: Transcription factor 3.

Table 2 The incidence of lung metastasis in nude mice following the transplantation of Hep3B and HCCLM3 cells with different treatment

	Lung metastasis
Hep3B-control	1/10
Hep3B-TCF3	5/10
HCCLM3-shcontrol	9/10
HCCLM3-shTCF3	4/10

HCC: Hepatocellular carcinoma; Sh: Short hairpin; TCF3: Transcription factor 3.

Table 3 The incidence of lung metastasis in nude mice following the transplantation of Hep3B-TCF3 and HCCLM3-shTCF3 cells with different treatment

	Lung metastasis
Hep3B-TCF3 + LV-shcontrol	6/10
Hep3B-TCF3 + LV-shMMP11	2/10
HCCLM3-shTCF3 + LV-control	3/10
HCCLM3-shTCF3 + LV-MMP11	7/10

HCC: Hepatocellular carcinoma; MMP11: Matrix metalloproteinase-11; Sh: Short hairpin; TCF3: Transcription factor 3.

Table 4 Correlation analysis of transcription factor 3 expression and matrix metallopeptidase 11 in human hepatocellular carcinoma tissues

		Transcription factor 3
		Positive (n = 35)	Negative (n = 41)
Matrix metalloproteinase-11	Positive (n = 30)	20	10	P = 0.004
	Negative (n = 46)	15	31