Maximizing pediatric stem cell harvest: A novel two-step digestion approach for adipose tissue

doi:10.4252/wjsc.v18.i3.116388

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Mar 26, 2026 (publication date) through Mar 28, 2026

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.

World J Stem Cells. Mar 26, 2026; 18(3): 116388
Published online Mar 26, 2026. doi: 10.4252/wjsc.v18.i3.116388

Table 1 Primers sequences used in gene expression analysis

Genes	Primer sequence 5’ → 3’
VEGF	F: TTGCCTTGCTGCTCTACCTCCA
VEGF	R: GATGGCAGTAGCTGCGCTGATA
SDF	F: CTCAACACTCCAAACTGTGCCC
SDF	R: CTCCAGGTACTCCTGAATCCAC
NOS3	F: GAAGGCGACAATCCTGTATGGC
NOS3	R: TGTTCGAGGGACACCACGTCAT
CD14	F: CTGGAACAGGTGCCTAAAGGAC
CD14	R: GTCCAGTGTCAGGTTATCCACC
MMP3	F: CACTCACAGACCTGACTCGGTT
MMP3	R: AAGCAGGATCACAGTTGGCTGG
IL4R	F: CTGCTCATGGATGACGTGGTCA
IL4R	R: GGTGTGAACTGTCAGGTTTCCTG
CDKN2A (P16)	F: CTCGTGCTGATGCTACTGAGGA
CDKN2A (P16)	R: GGTCGGCGCAGTTGGGCTCC
P53	F: CCTCAGCATCTTATCCGAGTGG
P53	R: TGGATGGTGGTACAGTCAGAGC
GAPDH	F: ACGGATTTGGTCGTATTGGG
GAPDH	R: CGCTCCTGGAAGATGGTGAT

VEGF: Vascular endothelial growth factor; SDF: Stromal cell-derived factor; NOS3: Nitric oxide synthase-3; MMP3: Matrix metalloproteinase 3; IL4R: Interleukin-4 receptor; CDKN2A: Cyclin-dependent kinase inhibitor 2A.

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Table 2 Comparative analysis of adipose tissue features across three sample types

Feature	Image 1	Image 2	Image 3
Adipocyte presence and size	Visible; small and densely packed	Visible; large and sparsely distributed	Not detectable or structurally degraded
Adipocyte integrity	Intact membranes	Somewhat deformed	Completely lysed or absent
Cell density	Dense regenerative cells	Moderate cell presence	Low regenerative cells (absence of adipocytes, only entrapped nuclei seen)
ECM visibility	Moderately apparent	Minimally visible	Highly apparent, rich in collagen content

ECM: Extracellular matrix.

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Citation: Mushtaq A, Choudhery MS, Arif T, Niaz A, Pervaiz A. Maximizing pediatric stem cell harvest: A novel two-step digestion approach for adipose tissue. World J Stem Cells 2026; 18(3): 116388
URL: https://www.wjgnet.com/1948-0210/full/v18/i3/116388.htm
DOI: https://dx.doi.org/10.4252/wjsc.v18.i3.116388