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©The Author(s) 2023.
World J Stem Cells. Aug 26, 2023; 15(8): 821-841
Published online Aug 26, 2023. doi: 10.4252/wjsc.v15.i8.821
Published online Aug 26, 2023. doi: 10.4252/wjsc.v15.i8.821
Table 1 Details of cardiac specific primers
| Gene | Primer sequences (5’-3’) | Annealing temperatures (ºC) | Product size (bp) |
| α-actinin (F) | 5’-CTCTTGCTTCTACCACGCTTT-3’ | 58.0 | 297 |
| α-actinin (R) | 5’-AGCGTGTTGAAGTTGATCTCC-3’ | ||
| cMHC (F) | 5’-GAGGAGCAAGCCAACACCA-3’ | 58.0 | 106 |
| cMHC (R) | 5’-GCAGCTTGTTGACCTGGGA-3’ | ||
| Mef2D (F) | 5’-GGGGGCTGGAGGAGTTACC-3’ | 58.0 | 292 |
| Mef2D (R) | 5’-TGGGGGAACGGTGTTGTCA-3’ | ||
| MLC-2v (F) | 5’-CCTGAGGAAACCATTCTCAACG-3’ | 58.0 | 98 |
| MLC-2v (R) | 5’-GATGTGCACCAGGTTCTTGTAG-3’ | ||
| cTnC (F) | 5’-CTCAACCCCAAATCCCCCGA-3’ | 58.0 | 148 |
| cTnC (R) | 5’-AGGAAGCGGCCATTGGGTAA-3’ | ||
| cTnI (F) | 5’-GAACATCACGGAGATTGCAGA-3’ | 58.0 | 236 |
| cTnI (R) | 5’-TCAGTGCATCGATGTTCTTCC-3’ | ||
| cTnT (F) | 5’-TCCAGAAGGCCCAGACAGAG-3’ | 58.0 | 89 |
| cTnT (R) | 5’-CACCTTCCTCCTCTCAGCCA-3’ | ||
| Ca- channel (F) | 5’-GAGAGCACCCCGGCTTC-3’ | 58.0 | 164 |
| Ca-channel (R) | 5’-GAAGTCCTGCCCCGCTC-3’ | ||
| Na-channel (F) | 5’-ACTAGGCAATTTGTCGGCTC-3’ | 58.0 | 325 |
| Na-channel (R) | 5’-GCCGTTCTTGAGCAGGTAAT-3’ | ||
| GATA4 (F) | 5’-CTGCCCTCCGTCTTCTGC-3’ | 58.0 | 286 |
| GATA4 (R) | 5’-CTCGCAGGTCAAGGAGCC-3’ | ||
| Nkx-2.5 (F) | 5’-CAAGTGTGCGTCTGCCTTTC-3’ | 58.0 | 106 |
| Nkx-2.5 (R) | 5’-CGCGCACAGCTCTTTCTTT-3’ | ||
| GAPDH (F) | 5′-CAC CAT GGG GAA GGT GAA GG-3’ | 58.0 | 274 |
| GAPDH (R) | 5′-AGC ATC GCC CCA CTT GAT TT-3’ |
Table 2 Details of Wnt pathway primers
| Genes | Primer sequences (5’-3’) | Annealing temperatures (ºC) | Product size (bp) |
| GAPDH (F) | 5′-CAC CAT GGG GAA GGT GAA GG-3’ | 58.0 | 274 |
| GAPDH (R) | 5′-AGC ATC GCC CCA CTT GAT TT-3’ | ||
| Wnt-2 (F) | 5’-GTCGGGAATCTGCCTTTGTT-3’ | 58.0 | 376 |
| Wnt-2 (R) | 5’-GTTTTCCTGAAGTCGGCCAT-3’ | ||
| DVL (F) | 5’CTATGGATCAGGATTTCGGGGT-3’ | 58.0 | 121 |
| DVL (R) | 5’-ATCTCGGGTTGGGGATTATCTG-3’ | ||
| GSK 3β (F) | 5’-TGTGTTGGCTGAGCTGTTACTA-3’ | 58.0 | 200 |
| GSK 3β (R) | 5’-TGAAATGTCCTGTTCCTGACGA-3’ | ||
| β-catenin (F) | 5’-TGATATTGGTGCCCAGGGAG-3’ | 58.0 | 102 |
| β-catenin (R) | 5’-TCCATACCCAAGGCATCCTG-3’ | ||
| c-myc (F) | 5’-CACTAACATCCCACGCTCTGA-3’ | 58.0 | 217 |
| c-myc (R) | 5’-CGCATCCTTGTCCTGTGAGTA-3’ | ||
| cyc-D (F) | 5’-CAGAGGCGGAGGAGAACAAA-3’ | 58.0 | 219 |
| cyc-D (R) | 5’-CCGGGTCACACTTGATCACT-3’ | ||
| c-jun (F) | 5’-TGAGCCTACAGATGAACTCTTTCT-3’ | 58.0 | 191 |
| c-jun (R) | 5’-ACTCAGAGTGCTCCAAATCTCTTA-3’ | ||
| TCF (F) | 5’-CGAGAAGAGCAGGCCAAGTA-3’ | 58.0 | 219 |
| TCF (R) | 5’-GAGCACTGTCATCGGAAGGA-3’ | ||
| Axin (F) | 5’-GCATGGAGGAGGAAGGTGAG-3’ | 58.0 | 165 |
| Axin (R) | 5’-CCAGGATGCTCTCAGGGTTC-3’ |
- Citation: Muneer R, Qazi REM, Fatima A, Ahmad W, Salim A, Dini L, Khan I. Wnt signaling pathway inhibitor promotes mesenchymal stem cells differentiation into cardiac progenitor cells in vitro and improves cardiomyopathy in vivo. World J Stem Cells 2023; 15(8): 821-841
- URL: https://www.wjgnet.com/1948-0210/full/v15/i8/821.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v15.i8.821