Exosomes derived from inflammatory myoblasts promote M1 polarization and break the balance of myoblast proliferation/differentiation

Table 1 Primary antibodies used in the experiment

Antibody	Source	Catalog No.	Type	Dilution	M.W. (kD)
CD63	Affinity	AF5117	Rabbit mAb	1:1000(W.B.)	47
CD9	Affinity	AF5139	Rabbit mAb	1:2000(W.B.)	23
Alix	Affinity	ab275377AF0184	Rabbit mAb	1:2000(W.B.)	95
HSP60	Affinity	AF0199	Rabbit mAb	1:2000(W.B.)	60
iNOS	Affinity		Rabbit mAb	1:1000(W.B.)	130
ARG1	Affinity	DF6657	Rabbit mAb	1:1000(W.B.)	35
CD86	Abcam	ab220188	Rabbit mAb	1:1000(W.B.)	38
				1:100(IF)
CD86 PE	eBioscience	12-0862-81	Rat mAb	0.125 μg/test	(Flow Cyt)
CD206	Affinity	DF4149	Rabbit mAb	1:1000(W.B.)	120
CD206	Abcam	ab64693	Rabbit mAb	1:500(IF)
	eBioscience	17-1631-80	Rat mAb	0.25 μg/test	(Flow Cyt)
CD163APC	Abcam	ab51263	Mouse mAb	1:1000(W.B.)	227
MYHC				1:500(IF)
MyoD1	Affinity	AF7733	Rabbit mAb	1:1000(W.B.)	60
MyoD1	Proteintech	18943-1-AP	Rabbit PAb	1:200(IF)
MyoG	Abcam	ab1835	Mouse mAb	1:1000(W.B.)	25
Collage 1	Affinity	AF7001	Rabbit mAb	1:1000(W.B.)	140
α-SMA	Affinity	AF1032	Rabbit mAb	1:1000(W.B.)	42
Tubulin	Affinity	T0023	Mouse mAb	1:1000(W.B.)	55
GAPDH	Affinity	AF7021	Rabbit mAb	1:1000(W.B.)	37
F4/80 APC	eBioscience	47-4801-80	Rat mAb	0.125 μg/test	(Flow Cyt)

α-SMA: α-smooth muscle actin.