Similarities and differences between mesenchymal stem/progenitor cells derived from various human tissues

Table 1 Antibodies used for immunofluorescence and for flow cytometry analysis

Antigen	Company		Dilution	Incubation time
Immunofluorescence staining
CD73	Thermo Fisher	Weston, Florida, USA	1:100	1 h
CD90	Abcam	Cambridge, UK	1:100	1 h
CD105	Dako	Palo Alto, California, USA	1:10	1 h
CD34			1:100	30 min
CD45			1:100	30 min
CD56			1:80	30 min
CD146	Santa Cruz	Dallas, Tx, USA	1:25	1 h
PDGFRα			1:25	1 h
PW1	Atlas Antibodies	Bromma, Sweden	1:100	1 h
Alexa Fluor			1:700	40 min
488 nm
Donkey anti-mouse
Alexa Fluor	Thermo Fisher	Weston, Florida, USA	1:700	40 min
488 nm
Donkey anti-rabbit
Alexa Fluor			1:700	40 min
594 nm
Donkey anti-mouse
Alexa Fluor			1:700	40 min
594 nm
Donkey anti-rabbit
Flow Cytometry
CD73 (IgG) APC	BD Pharmingen	San Jose, California, USA	1:25	30 min
CD90 (IgG) FITC			1:25	30 min
CD105 (IgG) PE			1:25	30 min
CD34 (IgG) FITC			1:25	30 min
CD45 (IgG) APC			1:25	30 min
CD56 (IgG) PE			1:25	30 min
CD146 (IgG) PE			1:25	30 min
Isotype controls:			1:25	30 min
IgG APC
IgG PE
IgG FITC

Table 2 Summary of biological properties of MSCs isolated from different tissues

Summary
Phenotype	Marker	BM-MSC			AT-MSC			SM-MSC			SK-MSC
		P1	P5	P10	P1	P5	P10	P1	P5	P10	P1	P5	P10
	CD73	+++	+++	+++	+++	+++	+++	+++	+++	+++	+++	+++	+++
	CD90	+++	+++	+++	+++	+++	++	+++	+++	++	+++	+++	+++
	CD105	+++	++	++	+++	++	+	++	++	++	+++	+++	++
	CD146	+++	++	++	+++	+	+/-	+	+	+/-	+	+	++
	PDGFRα	++	++	+/-	+++	+	+/-	++	+	+	+	+	+
	PW1	-	+/-	+/-	++	+/-	+/-	+++	+/-	+/-	++	+/-	+/-
Differentiation	Tissue	BM-MSC			AT-MSC			SM-MSC			SK-MSC
		P1	P5	P10	P1	P5	P10	P1	P5	P10	P1	P5	P10
	Osteo-	++	++	+++	+	++	+++	++	++	++	+	++	+++
	Adipo-	+++	++	+	+++	++	++	-	-	-	+	++	+/-
	Chondro-	++	++	+++	++	++	+++	+	++	++	+	++	++
mRNA ecpression	Gene	BM-MSC			AT-MSC			SM-MSC			SK-MSC
		P1	P5	P10	P1	P5	P10	P1	P5	P10	P1	P5	P10
	Sox2	+	++	+++	+	+	+	+	+	+	+++	+	+
	Oct4	+	++	+++	+	+	+	++	+/-	+	+	+	+
	p53	+	++	++	++	++	++	++	+	++	++	++	+
	c-myc	+	+++	++	+++	++	+++	++	++	++	++	++	++
Cytokines and chemokines	Cytokine	BM-MSC			AT-MSC			SM-MSC			SK-MSC
		P1	P5	P10	P1	P5	P10	P1	P5	P10	P1	P5	P10
	MCP-1	+	++	++	+/-	+	+	+	++	+	+	+	+
	IL-8	+	+++	+++	+/-	+	++	-	-	++	+	+	+
	VEGF	+++	+++	+	-	+	+/-	+	+	+/-	-	+/-	-
	IL-5	+/-	+/-	+/-	+/-	+/-	+/-	+/-	+/-	+/-	-	-	-
	IFN-γ	+/-	+	+/-	+/-	+/-	+	+/-	+	+/-	+/-	+/-	+/-
	IL-6	+/-	+	+	+++	+++	++	+/-	+	+	-	+/-	-
	MIP-1β	+	+	+	+	+	+	-	+/-	+	+/-	+/-	+/-

The gradation reflects the changes in the value of the examined parameters compared to passage 1: (-) negative, (+/-) weak, (+) moderate positive, (++) positive, (+++) strong positive.