Published online Jun 26, 2019. doi: 10.4252/wjsc.v11.i6.347
Peer-review started: October 27, 2018
First decision: November 15, 2018
Revised: December 3, 2018
Accepted: January 26, 2019
Article in press: January 26, 2019
Published online: June 26, 2019
Processing time: 242 Days and 17.1 Hours
Mesenchymal stromal/stem cells (MSCs) are applied in experimental clinical procedures as a promising tool in regenerative medicine. Cells with basic MSCs characteristics can be isolated from different human tissues. However, their biological properties are still not fully cha-racterized. Although MSCs from different tissues exhibit many common characteristics, some markers and biological properties are different and depend on their tissue of origin.
The biological diversity of MSCs, depending on their original tissue location, methodology of isolation, and culture conditions encouraged us to explore the biological properties of MSCs of different tissue-origin in long-term in vitro culture. Recognizing the activity of factors that underlie MSC biology should constitute important points for consideration before clinical MSC application.
In this study, we characterize the biological properties of MSCs during longterm culture isolated from: bone marrow (BM-MSCs), adipose tissue (AT-MSCs), skeletal muscles (SM-MSCs), and skin (SK-MSCs).
MSCs were isolated from the examined tissues and cultured up to 10 passages. MSCs were assessed for: phenotype with immunofluorescence and flow cytometry, multipotency with differentiation capacity for osteo-, chondro-, and adipogenesis, stemness markers with qPCR for mRNA for Sox2 and Oct4, and genetic stability for p53 and c-Myc. Furthermore, 27 bioactive factors were screened with the multiplex ELISA array, and spontaneous fusion involving a co-culture of SM-MSCs with BM-MSCs or AT-MSCs stained with PKH26 (red) or PKH67 (green) was carried out.
All examined MSCs showed the basic MSC phenotype CD73, CD90, CD105 stable up to P10. However, their expression decreased with the age of culture, as confirmed by fluorescence intensity. The proangiogenic properties of MSCs were confirmed by CD146 expression, however, long-term culture is unfavorable for maintaining the proangiogenic function of examined MSCs, but not for BM-MSCs. All examined MSCs, except BM-MSCs, expressed PW1, a marker associated with differentiation capacity and apoptosis. BMMSCs and ATMSCs expressed the stemness markers Sox2 and Oct4 in long-term culture. All examined MSCs were stable in terms of p53 and c-Myc expression. The differentiation capacity of BM-MSCs and AT-MSCs was maintained during the follow-up period. In contrast, SK-MSCs and SM-MSCs had a limited ability to differentiate into adipocytes. BM-MSCs and AT-MSCs revealed similarities in phenotype maintenance, the ability to undergo multilineage differentiation, and secretion of bioactive factors. The fact that AT-MSCs fused with SM-MSCs as effectively as BM-MSCs indicates that AT-MSCs may serve as an alternative source for BM-MSCs.
Long-term culture affects the biological activity of MSCs obtained from various tissues. The source of MSCs with specific biological properties and the duration of the culture will be an important consideration for their selection for regenerative medicine.
Knowledge of MSC biology is developing, but remains incomplete, and there is still much room for exploration in basic in vitro and in vivo research before MSCs can be used in therapy.