Kozlowska U, Krawczenko A, Futoma K, Jurek T, Rorat M, Patrzalek D, Klimczak A. Similarities and differences between mesenchymal stem/progenitor cells derived from various human tissues. World J Stem Cells 2019; 11(6): 347-374 [PMID: 31293717 DOI: 10.4252/wjsc.v11.i6.347]
Corresponding Author of This Article
Aleksandra Klimczak, DSc, PhD, Associate Professor, Laboratory of Biology of Stem and Neoplastic Cells, Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences, Rudolfa Weigla 12 Street, Wroclaw 53-114, Poland. aleksandra.klimczak@hirszfeld.pl
Research Domain of This Article
Cell Biology
Article-Type of This Article
Basic Study
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Kozlowska U, Krawczenko A, Futoma K, Jurek T, Rorat M, Patrzalek D, Klimczak A. Similarities and differences between mesenchymal stem/progenitor cells derived from various human tissues. World J Stem Cells 2019; 11(6): 347-374 [PMID: 31293717 DOI: 10.4252/wjsc.v11.i6.347]
World J Stem Cells. Jun 26, 2019; 11(6): 347-374 Published online Jun 26, 2019. doi: 10.4252/wjsc.v11.i6.347
Similarities and differences between mesenchymal stem/progenitor cells derived from various human tissues
Urszula Kozlowska, Agnieszka Krawczenko, Katarzyna Futoma, Tomasz Jurek, Marta Rorat, Dariusz Patrzalek, Aleksandra Klimczak
Urszula Kozlowska, Agnieszka Krawczenko, Katarzyna Futoma, Aleksandra Klimczak, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw 53-114, Poland
Tomasz Jurek, Marta Rorat, Department of Forensic Medicine, Wroclaw Medical University, Wroclaw 50-345, Poland
Dariusz Patrzalek, Faculty of Health Science, Department of Physiotherapy, Wroclaw Medical University, Wroclaw 50-367, Poland
Author contributions: Kozlowska U performed the majority of experiments, analyzed the data, and wrote the paper; Krawczenko A, and Futoma K contributed to experiment preparation and acquisition and interpretation of data; Jurek T, Rorat M, and Patrzalek D contributed to material and data acquisition; Klimczak A designed the study, analyzed data, and wrote the paper.
Supported bythe National Science Center, No. N407121940; and by the Wroclaw Centre of Biotechnology, the Leading National Research Centre (KNOW) program for the years 2014-2018.
Institutional review board statement: Research was performed using human samples and was approved by the institutional review board of the Bioethics Committee of Wroclaw Medical University No. KB-746/2012 and No. KB 201/2016.
Institutional review board statement: The Institutional Animal Care and Use Committee Approval Form is not applicable for the manuscript. Studies were not performed on animal models.
Conflict-of-interest statement: The authors declare that there is no conflict of interests regarding the publication of this paper.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The ARRIVE Guidelines Checklist is not applicable for the manuscript, studies were not performed on animal models.
Corresponding author: Aleksandra Klimczak, DSc, PhD, Associate Professor, Laboratory of Biology of Stem and Neoplastic Cells, Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences, Rudolfa Weigla 12 Street, Wroclaw 53-114, Poland. aleksandra.klimczak@hirszfeld.pl
Telephone: +48-71-3371172 Fax: +48-71-3372171
Received: October 27, 2018 Peer-review started: October 27, 2018 First decision: November 15, 2018 Revised: December 3, 2018 Accepted: January 26, 2019 Article in press: January 26, 2019 Published online: June 26, 2019 Processing time: 242 Days and 17.1 Hours
Core Tip
Core tip: A comprehensive characterization of mesenchymal stromal/stem cells (MSCs) with different tissue origin during long-term culture was demonstrated in terms of: basic phenotype strength, stemness and genetic stability, and ability to secrete bioactive factors and affect one another in co-culture. MSCs were phenotypically heterogeneous and showed diverse differentiation potentials and secretion of bioactive factors associated with tissue origin. Bone marrow (BM)-MSCs and adipose tissue (AT)-MSCs expressed stemness markers Sox2 and Oct4 in long-term culture, whereas skeletal muscles (SM)-MSCs and skin (SK)-MSCs did not. All MSCs were stable for p53 and c-Myc expression. AT-MSCs fused with SM-MSCs as effectively as BM-MSCs. Long-term culture affected the biological properties of the MSCs.
