Correction to “ADSC-Exos outperform BMSC-Exos in alleviating hydrostatic pressure-induced injury to retinal ganglion cells by upregulating nerve growth factors”

Figure 1 Adipose-derived stem cells, bone marrow-derived stem cell-exosomes, and retinal ganglion cells were isolated and characterized. A: Immunofluorescence staining of the stem cell marker SOX2 (scale bar: 20 μm); B: Electron microscopy images of the morphology of adipose-derived stem cell-exosomes and bone marrow-derived stem cell-exosomes (scale bar: 200 nm); C: Adipose-derived stem cell-exosomes and bone marrow-derived stem cell-exosomes marker molecules (CD9, CD63, and CD81) were detected by western blotting; D: Light microscopy images of retinal ganglion cells (magnification: 40 × and 400 × ); E: Isolated retinal ganglion cells were examined by Thy1.1 immunofluorescence (scale bar: 50 μm). ADSC: Adipose-derived stem cell; BMSC: Bone marrow-derived stem cell; Exo: Exosome.

Figure 2 Growth of retinal ganglion cells. A: Phase contrast microscopy images of the morphology and growth of retinal ganglion cells (scale bar: 50 μm); B: Transmission electron microscopy images of retinal ganglion cell morphology (scale bar: 5 μm). ADSC: Adipose-derived stem cell; BMSC: Bone marrow-derived stem cell; Exo: Exosome; NC: Normal control.

Figure 3 Immunofluorescence detection of β-III tubulin. A-C: Immunofluorescence staining of β-III tubulin (red) and nuclear staining with DAPI (blue) (left panel) and the number of β-III tubulin-positive retinal ganglion cells (right panel) in different groups after exposure to 40 mmHg (A), 80 mmHg (B), and 120 mmHg (C). ^aP < 0.05, compared with the normal control group (scale bar: 100 μm); ^bP < 0.05, compared with the model (40, 80, and 120 mmHg) group. ADSC: Adipose-derived stem cell; BMSC: Bone marrow-derived stem cell; Exo: Exosome; NC: Normal control.

Figure 4 Immunofluorescence detection of p-CREB and CREB. A-C: Immunofluorescence staining of CREB (green) and p-CREB (red) and nuclear staining with DAPI (blue) (left panel) and quantification of the p-CREB/CREB ratio (right panel) in different groups after exposure to (A) 40 mmHg, (B) 80 mmHg, and (C) 120 mmHg (scale bar: 20 μm). ^aP < 0.05, compared with the normal control group; ^bP < 0.05, compared with the model (40, 80, and 120 mmHg) group. ADSC: Adipose-derived stem cell; BMSC: Bone marrow-derived stem cell; Exo: Exosome; NC: Normal control.