Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.
World J Stem Cells. May 26, 2026; 18(5): 114668
Published online May 26, 2026. doi: 10.4252/wjsc.v18.i5.114668
Published online May 26, 2026. doi: 10.4252/wjsc.v18.i5.114668
Isolation and proteomic profiling of mesenchymal stem cell-derived extracellular vesicles: Unlocking new frontiers in regenerative and translational medicine
Mir Sadat-Ali, Department of Orthopedic Surgery, Dallah Hospital, AlKhobar 31952, Saudi Arabia
Haifa A Al-Turki, Department of Obstetrics and Gynecology, Art-Haifa Fertility Clinics, AlKhobar 31142, Saudi Arabia
Haifa A Al-Turki, Assisted Reproductive Technology, Art-Haifa Fertility Clinics, AlKhobar 32424, Saudi Arabia
Aleem A Khan, Central Laboratory for Stem Cell and Translational Medicine, Deccan College of Medical Sciences, Hyderabad 500089, Telangana, India
Saira M Bannu, Stem Cell Regenerative Medicine, StemCells Regenerative and Research Laboratory, Hyderabad 500035, Telangāna, India
Shivaranjani Vutharadhi, Stem Cell Laboratory, StemCells Regenerative and Research Labs, Hyderabad 500034, Telangāna, India
Mirza M Baig, Research and Innovations, CMH Research and Innovations, Secunderabad 500011, Telangāna, India
Co-first authors: Mir Sadat-Ali and Haifa A Al-Turki.
Author contributions: Sadat-Ali M and Al-Turki HA contributed equally to this manuscript and are co-first authors. Sadat-Ali M, Al-Turki HA, and Khan A contributed to conceptualization and design; Sadat-Ali M and Khan A contributed to administrative support; Sadat-Ali M and Al-Turki HA contributed to provision of study materials or patients; Bannu SM, Vutharadhi S, and Baig MM contributed to data collection and assembly; Sadat-Ali M, Khan AA, Bannu SM, and Baig MM contributed to data analysis and interpretation. All authors contributed to manuscript writing and approved the final manuscript.
Institutional review board statement: The study was approved by the IRB of StemCells Regenerative and Research Labs (No. 10296/2025).
Conflict-of-interest statement: The authors report no relevant conflicts of interest for this article.
Data sharing statement: The data is available with the corresponding author and will be provided on request.
Corresponding author: Mir Sadat-Ali, Professor, Department of Orthopedic Surgery, Dallah Hospital, Pobox 40071, AlKhobar 31952, Saudi Arabia. drsadat@hotmail.com
Received: September 26, 2025
Revised: November 25, 2025
Accepted: March 5, 2026
Published online: May 26, 2026
Processing time: 242 Days and 15.4 Hours
Revised: November 25, 2025
Accepted: March 5, 2026
Published online: May 26, 2026
Processing time: 242 Days and 15.4 Hours
Core Tip
Core Tip: In regenerative medicine, mesenchymal stem cell (MSC)-based therapy is used to repair diseased or damaged tissue. The technology of culturing MSCs is expensive and not available everywhere. MSC-derived extracellular vesicles have gained attention as a potential therapeutic alternative to MSCs themselves. Therefore, scientists should be able to isolate and identify individual proteins and their functionality, which can be useful for therapeutic purposes. We used proteomics to isolate and identify 53 proteins from MSC-derived extracellular vesicles, 15 of which can be targeted in the field of regenerative medicine.