Wang K, Lin W, Han JY, Chen JY, Liu RH, Yu Z, Jin JJ. Differentiation of patient-specific induced pluripotent stem cells derived from type 1 diabetes peripheral blood mononuclear cells into pancreatic β-like cells. World J Stem Cells 2025; 17(7): 104607 [PMID: 40740538 DOI: 10.4252/wjsc.v17.i7.104607]
Corresponding Author of This Article
Kun Wang, PhD, Associate Professor, Fujian Key Laboratory of Medical Analysis, Fujian Academy of Medical Sciences, No. 7 Wusi Road, Fuzhou 350001, Fujian Province, China. wangkun973@fjms.ac.cn
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Cell & Tissue Engineering
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Basic Study
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Jul 26, 2025 (publication date) through Feb 15, 2026
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World Journal of Stem Cells
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1948-0210
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Wang K, Lin W, Han JY, Chen JY, Liu RH, Yu Z, Jin JJ. Differentiation of patient-specific induced pluripotent stem cells derived from type 1 diabetes peripheral blood mononuclear cells into pancreatic β-like cells. World J Stem Cells 2025; 17(7): 104607 [PMID: 40740538 DOI: 10.4252/wjsc.v17.i7.104607]
World J Stem Cells. Jul 26, 2025; 17(7): 104607 Published online Jul 26, 2025. doi: 10.4252/wjsc.v17.i7.104607
Differentiation of patient-specific induced pluripotent stem cells derived from type 1 diabetes peripheral blood mononuclear cells into pancreatic β-like cells
Kun Wang, Wei Lin, Jun-Yong Han, Jin-Yan Chen, Rong-Hua Liu, Zhen Yu, Jing-Jun Jin
Kun Wang, Jun-Yong Han, Jin-Yan Chen, Rong-Hua Liu, Zhen Yu, Jing-Jun Jin, Fujian Key Laboratory of Medical Analysis, Fujian Academy of Medical Sciences, Fuzhou 350001, Fujian Province, China
Wei Lin, Department of Endocrinology, Shengli Clinical College of Fujian Medical University, Fuzhou University Affiliated Provincial Hospital, Fuzhou 350001, Fujian Province, China
Author contributions: Wang K and Lin W conceived of the project and wrote the manuscript; Han JY, Chen JY, and Jin JJ also contributed to study conception and design; Lin W performed sample collection and analysis; Wang K, Han JY, Liu RH, and Yu Z performed material preparation, data analysis, and conducted most of the experiments; Chen JY and Jin JJ provided critical discussions and advice; Wang K and Han JY edited the manuscript. All authors have read and agreed to the published version of the manuscript.
Supported by the Nonprofit Research Institutes Foundation of Fujian Province, China, No. 2020R1011003 and No. 2022R1012001; and the Talents Training Project for the Key Young Scholars of Fujian Provincial Health Commission, China, No. 2021GGA056.
Institutional review board statement: The study was reviewed and approved by the Ethics Committee of Fujian Provincial Hospital (approval number: K2021 04 020).
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Ethics Committee of Fujian Academy of Medical Sciences (approval number: DL2021-05).
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: No additional data are available.
Corresponding author: Kun Wang, PhD, Associate Professor, Fujian Key Laboratory of Medical Analysis, Fujian Academy of Medical Sciences, No. 7 Wusi Road, Fuzhou 350001, Fujian Province, China. wangkun973@fjms.ac.cn
Received: December 28, 2024 Revised: March 26, 2025 Accepted: June 18, 2025 Published online: July 26, 2025 Processing time: 210 Days and 0.3 Hours
Core Tip
Core Tip: This study successfully employed an episomal plasmid system to reprogram patient peripheral blood mononuclear cells into type 1 diabetes (T1D)-induced pluripotent stem cells (iPSCs). Optimization of the differentiation protocol, utilizing triiodothyronine (T3), vitamin C (Vc), and adenovirus-M3C, facilitated the directed differentiation of T1D-iPSCs toward pancreatic β-cells. The T3 + Vc group exhibited minimal neurogenin 3 expression, while the M3C + T3 + Vc group demonstrated maximal MAF bZIP transcription factor A expression. While insulin⁺ β-like cells differentiated from T1D-iPSCs displayed basal insulin secretion, they lacked the ability to respond to glucose stimulation, indicating functional immaturity compared to fully mature β-cells. These results underscore their potential as seed cells for β-cell replacement therapy in T1D.
