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©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Nov 26, 2025; 17(11): 112484
Published online Nov 26, 2025. doi: 10.4252/wjsc.v17.i11.112484
Published online Nov 26, 2025. doi: 10.4252/wjsc.v17.i11.112484
HIF-1α modulates β-catenin pathway to enhance the survival and angiogenesis of PBMSCs under hypoxia environment
Peng-Zhen Wang, Ping-Ping Zhu, Wei Yuan, Jian Wu, Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital of Jinan University, Guangzhou 510220, Guangdong Province, China
Ai-Qun Wang, Department of Anesthesiology, Guangzhou Red Cross Hospital of Jinan University, Guangzhou 510000, Guangdong Province, China
Ping-Ge Tian, Department of Cardiology, Guangzhou Red Cross Hospital of Jinan University, Guangzhou 510000, Guangdong Province, China
Rui Zhang, Department of Emergency, Guangzhou Red Cross Hospital of Jinan University, Guangzhou 510220, Guangdong Province, China
Co-first authors: Peng-Zhen Wang and Ai-Qun Wang.
Author contributions: Wang PZ, Wang AQ, Zhang R, Zhu PP, Yuan W, and Wu J performed all experiments; Wang PZ, Wang AQ, Tian PG, Zhu PP, and Yuan W participated in the data collection and data analysis; Wang PZ and Zhang R supervised the study and edited the manuscript; Wang PZ, Tian PG, and Zhang R acquired the funding. All the authors have read and approved the final manuscript. Wang PZ and Wang AQ contributed equally to this manuscript and are co-first authors.
Supported by Guangdong Provincial Natural Science Foundation General Program, No. 2025A1515012455; and Guangzhou Municipal Science and Technology Project, No. 2024A03J0653, No. 2023A03J0595, No. 2023A03J0510, and No. 2023A03J0528.
Institutional animal care and use committee statement: All animal experimental procedures were performed in adherence with the Guide for the Care and Use of Laboratory Animals published by the United States National Institutes of Health (revised 1996) and with the approval of the Animal Care and Use Committee of Guangzhou Red Cross Hospital (Approval No. 2023-027-1). All surgery and euthanasia were performed under sodium pentobarbital anesthesia (100 mg/kg) by intraperitoneal injection, and all efforts were made to minimize suffering. This study did not involve human subjects and human cells.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: The datasets used or/and analyzed during the current study are available from the corresponding author on reasonable request.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Rui Zhang, MD, Chief Physician, Department of Emergency, Guangzhou Red Cross Hospital of Jinan University, No. 396 Tongfu Middle Road, Guangzhou 510220, Guangdong Province, China. 63281796@qq.com
Received: July 28, 2025
Revised: September 2, 2025
Accepted: October 9, 2025
Published online: November 26, 2025
Processing time: 120 Days and 19.2 Hours
Revised: September 2, 2025
Accepted: October 9, 2025
Published online: November 26, 2025
Processing time: 120 Days and 19.2 Hours
Core Tip
Core Tip: The finding of this study indicates that hypoxia-inducible factor-1α (HIF-1α) and β-catenin facilitate peripheral blood mesenchymal stem cells (PBMSCs) self-renewal and inhibit PBMSCs apoptosis. In vivo, PBMSCs with upregulated HIF-1α achieved enhanced retention within infarcted myocardial and contributed markedly to myocardial regeneration. These observations highlight the modulatory role of HIF-1α in modulating PBMSCs function via β-catenin-dependent anti-apoptotic and pro-angiogenic pathway, thereby offering a potential strategy to improve the longevity and therapeutic efficacy of PBMSCs.