Wang MF, Zhao SS, Thapa DM, Song YL, Xiang Z. Metabolomics of Fuzi-Gancao in CCl4 induced acute liver injury and its regulatory effect on bile acid profile in rats. World J Gastroenterol 2021; 27(40): 6888-6907 [PMID: 34790013 DOI: 10.3748/wjg.v27.i40.6888]
Corresponding Author of This Article
Mo-Fei Wang, PhD, Professor, The Second Department of General Surgery, The Affiliated Hospital of Inner Mongolia University for the Nationalities, No. 1742 East Section of Huolinhe Street, Tongliao 028000, Inner Mongolia Autonomous Region, China. wangm1228@sina.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Mo-Fei Wang, Dil Momin Thapa, Yu-Ling Song, The Second Department of General Surgery, The Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao 028000, Inner Mongolia Autonomous Region, China
Song-Song Zhao, Department of Educational Administration, The First Affiliated Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China
Zheng Xiang, School of Pharmaceutical Science, Liaoning University, Shenyang 110032, Liaoning Province, China
Author contributions: Xiang Z and Wang MF designed and coordinated the study; Wang MF, Thapa DM, and Song YL performed the experiments and acquired and analyzed the data; Wang MF and Zhao SS wrote the manuscript; all authors approved the final version of the article.
Supported byScientific Research Projects for Higher Education in Inner Mongolia Autonomous Region, No. NJZZ21027; Support Plan for the Innovation and Entrepreneurship Initiation Plan for Overseas Students in Inner Mongolia Autonomous Region, No. MOHRSS2020122; Doctoral Start-up Fund of the Affiliated Hospital of Inner Mongolia University for the Nationalities, No. MDFY2020001.
Institutional animal care and use committee statement: All animal experiments conformed to the internationally accepted principles for the care and use of laboratory animals. The study was reviewed and approved by the Liaoning University Research Ethics Committee.
Conflict-of-interest statement: There are no conflicts of interest to disclose.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Mo-Fei Wang, PhD, Professor, The Second Department of General Surgery, The Affiliated Hospital of Inner Mongolia University for the Nationalities, No. 1742 East Section of Huolinhe Street, Tongliao 028000, Inner Mongolia Autonomous Region, China. wangm1228@sina.com
Received: April 17, 2021 Peer-review started: April 17, 2021 First decision: June 23, 2021 Revised: June 28, 2021 Accepted: August 25, 2021 Article in press: August 25, 2021 Published online: October 28, 2021 Processing time: 193 Days and 4.2 Hours
ARTICLE HIGHLIGHTS
Research background
Fuzi (Radix aconiti lateralis)-Gancao (Radix glycyrrhizae) (F-G) is often used in the treatment of liver diseases such as hepatitis and liver failure.
Research motivation
This study can clarify the bile acid mechanism of F-G in the treatment of liver injury, and establish a complete bile acid spectrum research method, so as to provide reference for future research.
Research objectives
To study the molecular mechanism and action mechanism of F-G in the treatment of liver injury, and to provide a theoretical basis for the clinical research of F-G.
Research methods
An ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of 92 metabolites from multiple pathways was established to explore the protective metabolic mechanism of F-G in serum on the liver.
Research results
A UPLC-MS/MS method for simultaneous determination of 11 bile acids was established to analyze the regulatory mechanism of F-G in serum. F-G decreased the contents of 11 bile acids in the serum in a dose-dependent manner.
Research conclusions
F-G could promote the conjugation of free bile acids to glycine and taurine, reduce the accumulation of free bile acids in the liver, regulate the compensatory degree of taurine, and decrease the content of taurine conjugated bile acids.
Research perspectives
The research group will continue to study the effect of bile acid metabolism regulation on molecular regulation in the body.
