Basic Research
Copyright ©The Author(s) 2002. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 15, 2002; 8(2): 363-366
Published online Apr 15, 2002. doi: 10.3748/wjg.v8.i2.363
Protective effect of estradiol on hepatocytic oxidative damage
Yan Liu, Ichiro Shimizu, Toshihiro Omoya, Susumu Ito, Xiao-Song Gu, Ji Zuo
Yan Liu, Ji Zuo, Department of Biology, Medical school of Fudan University, Fudan University, Shanghai, 210032, China
Ichiro Shimizu, Toshihiro Omoya, Susumu Ito, Second Department of Internal Medicine, School of Medicine, University of Tokushima, Tokushima, 770-8503, Japan
Yan Liu, Xiao-Song Gu, Department of Biology, Nantong Medical College, Nantong, 226001, Jiangsu Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Yan Liu, Department of Biology, Medical School of Fudan University, 130 Dongan Road, Shanghai, 210032, China. liya9899@yahoo.com
Telephone: +86-21-64041900-2311
Received: August 9, 2001
Revised: August 15, 2001
Accepted: August 23, 2001
Published online: April 15, 2002
Abstract

AIM: To examine the protective effect of estradiol on the cultured hepatocytes under oxidative stress.

METHODS: Hepatocytes of rat were isolated by using perfusion method, and oxidative stress was induced by a serum-free medium and FeNTA. MDA level was determined with TBA method. Cell damage was assessed by LDH assay. Apoptosis of hepatocytes was assessed with cytoflowmetric analysis. Expression of Bcl-xl in cultured hepatocytes was detected by Western blot. The radical-scavenging activity of estradiol was valued by its ability to scavenge the stable free radical of DDPH.

RESULTS: Oxidative stress increased LDH (from 168 ± 25 × 10-6 IU•cell-1 to 780 ± 62 × 10-6 IU•cell-1) and MDA (from 0.28 ± 0.07 × 10-6 nmol·cell-1 to 1.35 ± 0.12 × 10-6 nmol•cell-1) levels in cultured hepatocyte, and estradiol inhibited both LDH and MDA production in a dose dependent manner. In the presence of estradiol 10-6 mol•L-1, 10-7 mol•L-1 and 10-8 mol•L-1, the LDH levels are 410 ± 53 × 10-6 IU•cell-1 (P < 0.01 vs oxidative group), 530 ± 37 × 10-6 IU•cell-1 (P < 0.01 vs oxidative group), 687 ± 42 × 10-6 IU•cell-1 (P < 0.05 vs oxidative group) respectively, and the MDA level are 0.71 ± 0.12 × 10-6 nmol•cell-1 (P < 0.01vs oxidative group), 0.97 ± 0.11 × 10-6 nmol•cell-1 (P < 0.01 vs oxidative group) and 1.27 ± 0.19 × 10-6 nmol•cell-1 respectively. Estradiol suppressed apoptosis of hepatocytes induced by oxidative stress, administration of estradiol (10-6 mol/L)decreased the apoptotic rate of hepatocytes under oxidative stress from 18.6% ± 1.2% to 6.5% ± 2.5%, P < 0.01. Bcl-xl expression was related to the degree of liver cell damage due to oxidative stress, and estradiol showed a protective action.

CONCLUSION: Estradiol protects hepatocytes from oxidative damage by means of its antioxidant activity.

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