Basic Research
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Jun 28, 2006; 12(24): 3854-3858
Published online Jun 28, 2006. doi: 10.3748/wjg.v12.i24.3854
Expression of macrophage inflammatory protein-1α in Kupffer cells following liver ischemia or reperfusion injury in rats
Wei Ma, Zuo-Ren Wang, Lei Shi, Yue Yuan
Wei Ma, Zuo-Ren Wang, Lei Shi, Department of Hepatobiliary Surgery, First Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China
Yue Yuan, Department of Pharmacy, First Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China
Co-first-author: Zuo-Ren Wang
Correspondence to: Dr. Wei Ma, Department of Hepatobiliary Surgery, First Hospital of Xi'an Jiaotong University, 1 Jiankang Road, Xi'an 710061, Shaanxi Province, China. wowotoumario@yahoo.com.cn
Telephone: +86-29-85324009 Fax: +86-29-85323201
Received: January 5, 2006
Revised: April 6, 2006
Accepted: April 14, 2006
Published online: June 28, 2006
Abstract

AIM: To explore the expression of macrophage inflammatory protein-1α (MIP-1α) in Kupffer cells (KCs) following liver ischemia/reperfusion injury IRI in rats.

METHODS: Forty male SD rats were divided randomly into five groups. A model of partial warm ischemia/reperfusion injury in the rat liver was established. KCs were isolated and incubated one hour, six hours, 12 h, and 24 h after the reperfusion. Tumor necrosis factor alpha (TNF-α) and interleukin-1beta (IL-1β) in the supernatants were measured by ELISA. MIP-1α in KCs was detected by immunocytochemical and RT-PCR.

RESULTS: No or few MIP-1α protein and mRNA were expressed in the KCs of the control group. Its expression in the IRI group had a significant increase after the reperfusion (P < 0.05), which was contrary to the control group.

CONCLUSION: The active behavior of the MIP-1α gene in KCs following liver ischemia/reperfusion injury is assumed to be one of the major causes for the hepatic ischemia/reperfusion injury.

Keywords: Liver; Ischemia/reperfusion; Kupffer cell; Macrophage inflammatory protein-1α