Association of the rs1333048 polymorphism in the ANRIL gene with different clinical forms of periodontitis: A meta-analysis

Table 1 Characteristics of included studies on rs1333048 polymorphisms in ANRIL gene and periodontitis

Reference	Ethnicity/country	Study design	Diagnosis (clinical subtypes)	Subject type	Sample size (Cc/Co)	Age (years) (Cc/Co)	HWE (P value)	Methods of genotyping	Score
Chen et al[10], 2012	Asian/China	Case/Control	CP	-	221/172	41.6 ± 8.8/40.0 ± 9.2	Yes	Real time PCR	14
Ernst et al[11], 2010	Caucasian/German and Northern Ireland	Case/Control	gAgP	-	130/339	15-35/40-80	Yes	Real time PCR	13
Schaefer et al[9], 2009	Caucasian/Germany	Verification/Replication	gAgP	-	151/736	38 ± 6.6/63 ± 7.4	Yes	SNPlex/TaqMan	14
			LAgP	-	137/368	37 ± 7.0/37 ± 9.6
Schaefer et al[12], 2011	Caucasian/Germany and Netherlands	Case/Control	CP	-	154/421	45.2 ± 9.5/32.3 ± 9.0	Yes	SNPlex/TaqMan	15
			AgP		159/421	33.8 ± 4.7/32.3 ± 9.0
Schaefer et al[20], 2013	Caucasian/Turkey, Italy and Germany	Exploration	AgP	Turkey	89/72	-	Yes	Array Immunochip	18
				Italy	44/99		Yes
				Germany	594/1441		Yes
Taiete et al[21], 2018	Mixed/Brazil	Case/Control	AgP	-	200/196	34.0 ± 4.6/30.5 ± 5.8	No	PCR-RFLP	17
			CP	-	190/196	50.0 ± 7.2/30.5 ± 5.8	Yes		16
Teeuw et al[22], 2015	Caucasian/Netherlands	Case/Control	Periodontitis	-	115/56	45.5 ± 9.9/43.9 ± 13.2	Yes	TaqMan

Cc: Case; Co: Control; HWE: Hardy-Weinberg Equilibrium, CP: Chronic periodontitis; gAgP: Generalized aggressive periodontitis; LAgP: Localized aggressive periodontitis; AgP: Aggressive periodontitis; PCR: Polymerase chain reaction.

Table 2 Meta-analysis calculation of the rs1333048 polymorphism in ANRIL gene and periodontitis risk (allelic and genotypic comparisons; subgroups analyses)

Comparison (n)	OR (95%CI)	P value (Z test)	I², %	Pheterogeneity	Statistical model used
Overall (n = 12)
M vs m	1.24 (1.15-1.34)	P < 0.05	28	P = 0.17	F
m vs M	0.80 (0.75-0.87)	P < 0.05	28	P = 0.17	F
MM vs mm	1.53 (1.32-1.78)	P < 0.05	30	P = 0.16	F
mm vs MM	0.65 (0.56-0.76)	P < 0.05	30	P = 0.16	F
MM vs Mm/mm	1.38 (1.22-1.56)	P < 0.05	27	P = 0.18	F
Mm vs MM/mm	0.97 (0.87-1.08)	P = 0.53	25	P = 0.20	F
CP (n = 3)
M vs m	1.25 (1.07-1.47)	P = 0.006	0	P = 0.81	F
m vs M	0.80 (0.68-0.94)	P = 0.006	0	P = 0.81	F
MM vs mm	1.58 (1.14-2.18)	P = 0.005	0	P = 0.77	F
mm vs MM	0.63 (0.46-0.87)	P = 0.005	0	P = 0.77	F
MM vs Mm/mm	1.36 (1.05-1.77)	P = 0.02	0	P = 0.80	F
Mm vs MM/mm	0.98 (0.79-1.23)	P = 0.89	0	P = 0.92	F
AgP (n = 8)
M vs m	1.23 (1.13-1.34)	P < 0.05	47	P = 0.07	F
m vs M	0.81 (0.75-0.89)	P < 0.05	47	P = 0.07	F
MM vs mm	1.49 (1.25-1.77)	P < 0.05	48	P = 0.06	F
mm vs MM	0.67 (0.57-0.80)	P < 0.05	48	P = 0.06	F
MM vs Mm/mm	1.37 (1.19-1.58)	P < 0.05	51	P = 0.05	F
Mm vs MM/mm	0.95 (0.84-1.08)	P = 0.44	49	P = 0.06	F
Caucasian (n = 9)
M vs m	1.27 (1.16-1.38)	P < 0.05	25	P = 0.22	F
m vs M	0.79 (0.72-0.86)	P < 0.05	25	P = 0.22	F
MM vs mm	1.59 (1.34-1.88)	P < 0.05	26	P = 0.21	F
mm vs MM	0.63 (0.53-0.74)	P < 0.05	26	P = 0.21	F
MM vs Mm/mm	1.39 (1.21-1.60)	P < 0.05	43	P = 0.08	F
Mm vs MM/mm	0.97 (0.87-1.08)	P = 0.94	33	P = 0.15	F
Mixed (n = 2)
M vs m	1.11 (0.91-1.36)	P = 0.55	68	P = 0.08	R
m vs M	0.90 (0.63-1.28)	P = 0.55	68	P = 0.08	R
MM vs mm	1.22 (0.83-1.81)	P = 0.55	70	P = 0.07	R
mm vs MM	0.82 (0.55-1.21)	P = 0.55	70	P = 0.07	R
MM vs Mm/mm	1.26 (0.93-1.72)	P = 0.14	0	P = 0.49	F
Mm vs MM/mm	0.84 (0.64-1.11)	P = 0.23	30	P = 0.23	F

OR: Odds ratio; CI: Confidence Intervals; I²: Heterogeneity index; M: Mutant allele; m: Wild type allele; F: Fixed-effect statistical model; R: Random-effects statistical model; CP: Chronic periodontitis; AgP: Aggressive periodontitis.