Richa Rattan, Rimesh Pal, Department of Endocrinology, Post Graduate Institute of Medical Education and Research, Chandigarh 160012, India
Parul Chawla Gupta, Department of Ophthalmology, Post Graduate Institute of Medical Education and Research, Chandigarh 160012, Punjab, India
Arvind Kumar Morya, Department of Ophthalmology, All India Institute of Medical Sciences, Hyderabad 508126, Telangana, India
Ripunjay Prasad, Department of Ophthalmology, RP Eye Institute, Delhi 110001, India
Author contributions: Morya AK, Rattan R, and Pal R designed the study; Rattan R, Pal R, Gupta PC, and Morya AK wrote the manuscript; Prasad R edited the manuscript; all of the authors read and approved the final version of the manuscript to be published.
Conflict-of-interest statement: All the authors declare that they have no conflict of interest.
Corresponding author: Arvind Kumar Morya, Doctor, MS, Neurosurgeon, Professor, Surgeon, Department of Ophthalmology, All India Institute of Medical Sciences, Bibi Nagar, Hyderabad 508126, Telangana, India. bulbul.morya@gmail.com
Received: March 2, 2024 Revised: September 22, 2024 Accepted: October 23, 2024 Published online: January 6, 2025 Processing time: 249 Days and 14.8 Hours
Core Tip
Core Tip: The letter highlights certain limitations of the study by Cao et al, which explored early second-trimester biomarkers, retinol-binding protein 4 and angiopoietin-like 8, for predicting gestational diabetes mellitus (GDM). While valuable, identifying GDM in the early second trimester may not provide sufficient time for pregnant mothers to adopt major lifestyle interventions. Early first-trimester prediction or pre-conceptional identification would be more clinically significant. While the study uses advanced proteomic techniques, including isobaric tags for relative and absolute quantitation and liquid chromatography-mass spectrometry, to identify differentially expressed proteins, it is constrained by a small sample size and methodological gaps. Specifically, the reliability of protein quantification and high-abundance protein removal is unclear, and the lack of experimental validation limits the functional interpretation of identified protein interactions.
