Observational Study
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Mar 6, 2022; 10(7): 2166-2173
Published online Mar 6, 2022. doi: 10.12998/wjcc.v10.i7.2166
In vitro maturation of human oocytes maintaining good development potential for rescue intracytoplasmic sperm injection with fresh sperm
Yun-Qiao Dong, Chuang-Qi Chen, Yu-Qiang Huang, Dun Liu, Xi-Qian Zhang, Feng-Hua Liu
Yun-Qiao Dong, Chuang-Qi Chen, Yu-Qiang Huang, Dun Liu, Xi-Qian Zhang, Feng-Hua Liu, Department of Assisted Reproductive Center, Guangdong Women and Children Hospital, Guangzhou 511400, Guangdong Province, China
Author contributions: Dong YQ and Liu FH contribute to conceptualization; Dong YQ and Liu D contribute to methodology; Dong YQ, Chen CQ, and Huang YQ contribute to formal analysis and investigation; Dong YQ, Liu FH, and Zhang XQ writing - original draft preparation; Dong YQ and Chen CQ writing - review and editing; Liu FH funding acquisition; Dong YQ and Zhang XQ resources; Liu FH and Zhang XQ supervision; all authors read and approved the final manuscript.
Supported by Science and Technology Collaborative Innovation Project of Guangzhou, No. 201704020217.
Institutional review board statement: The study was reviewed and approved by the [Guangdong Women and Children’s Hospital] Institutional Review Board [Approval No. 202001158].
Informed consent statement: Informed consent was obtained from all individual participants included in the study.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
Data sharing statement: No additional data are available.
STROBE statement: The authors have read the STROBE Statement—checklist of items, and the manuscript was prepared and revised according to the STROBE Statement—checklist of items.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Feng-Hua Liu, MD, Doctor, Department of Assisted Reproductive Center, Guangdong Women and Children Hospital, No. 521 Xingnan Avenue, Guangzhou 511400, Guangdong Province, China. liushine200606@126.com
Received: July 27, 2021
Peer-review started: July 27, 2021
First decision: December 17, 2021
Revised: December 24, 2021
Accepted: January 25, 2022
Article in press: January 25, 2022
Published online: March 6, 2022
Processing time: 217 Days and 16.2 Hours
Core Tip

Core Tip: Owing to the low implantation rate, in vitro maturation (IVM) is difficult to widely adopt in clinical practice. Therefore, there is an urgent need to explore methods for improving the clinical outcome of IVM. We studied the effect of sperm on early embryo development from in vitro-matured oocytes. Our results show that IVM with conventional culture medium combined with the use of fresh sperm collected on the day of rescue intracytoplasmic sperm injection is an easy-to-implement strategy for patients whose oocytes are completely or mostly immature.