Mucin 1 and interleukin-11 protein expression and inflammatory reactions in the intestinal mucosa of necrotizing enterocolitis children after surgery

doi:10.12998/wjcc.v9.i25.7372

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 9, Issue 25

This Article

Peer-Review Report of This Article

CrossCheck and Google Search of This Article

Academic Rules and Norms of This Article

Citation of this article

Corresponding Author of This Article

Research Domain of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (6499)

All Articles published online

The chart showing PDF series, WORD series, HTML series, Figures (1-3) series, Tables (1-5) series.

Item

Count

PDF

328

WORD

231

HTML

2396

Figures (1-3)

1058

Tables (1-5)

500

Sum=4513

Publishing Process of This Article

The chart showing Browse series, Download series.

Item

Count

Browse

496

Download

1491

Sum=1987

Sep 6, 2021 (publication date) through Mar 2, 2026

Times Cited of This Article

Times Cited (6)

Journal Information of This Article

Publication Name

World Journal of Clinical Cases

ISSN

2307-8960

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

- Full Article with Cover (PDF)
- Full Article (XML)

Retrospective Study

World J Clin Cases. Sep 6, 2021; 9(25): 7372-7380
Published online Sep 6, 2021. doi: 10.12998/wjcc.v9.i25.7372

Mucin 1 and interleukin-11 protein expression and inflammatory reactions in the intestinal mucosa of necrotizing enterocolitis children after surgery

Hong-Xia Pan, Chang-Song Zhang, Chia-Hui Lin, Min-Min Chen, Xiao-Zhong Zhang, Nong Yu

Hong-Xia Pan, Min-Min Chen, Department of Clinical Laboratory Medicine, Suzhou BenQ Medical Center, Affiliated BenQ Hospital of Nanjing Medical University, Suzhou 215011, Jiangsu Province, China

Chang-Song Zhang, Department of Clinical Laboratory Medicine, The Affiliated Suzhou Science and Technology Town Hospital, Nanjing Medical University, Suzhou 215153, Jiangsu Province, China

Chia-Hui Lin, General Manager's Office, Suzhou Gallant Biotech Biotechnology Co. Ltd, Suzhou 215163, Jiangsu Province, China

Xiao-Zhong Zhang, Department of Cardiology, National High Tech Development Zone Hospital, Suzhou 215129, Jiangsu Province, China

Nong Yu, Department of Laboratory Medicine, National High Tech Development Zone Hospital, Suzhou 215129, Jiangsu Province, China

Author contributions: Pan HX and Zhang CS have the same contribution to this article and should be regarded as co-first authors; Pan HX and Zhang CS design the experiment; Lin CH drafted the work, Chen MM, Zhang XZ and Yu N collected the data; Pan HX and Zhang CS interpreted data; Pan HX, Zhang CS and Yu N wrote the article.

Supported by Suzhou Science and Technology Program, No. SLT202005; Suzhou Municipal Commission of Health and Family Planning, No. LCZX202031; Suzhou New District Science and Technology Plan, No. 2019Z009; and Independent Innovation Project of National High Tech Development Zone Hospital, No. SGY2018C03.

Institutional review board statement: This study was approved by the Affiliated BenQ Hospital of Nanjing Medical University Ethics Committee.

Informed consent statement: All study participants, or their legal guardian, provided informed written consent prior to study enrollment.

Conflict-of-interest statement: No conflict of interest.

Data sharing statement: No additional data are available.

Corresponding author: Nong Yu, BM BCh, Chief Technician, Department of Laboratory Medicine, National High Tech Development Zone Hospital, No. 95 Huashan Road, Huqiu District, Suzhou 215129, Jiangsu Province, China. yunong78612@sina.com

Received: April 22, 2021
Peer-review started: April 22, 2021
First decision: May 24, 2021
Revised: June 28, 2021
Accepted: July 28, 2021
Article in press: July 28, 2021
Published online: September 6, 2021
Processing time: 130 Days and 22.1 Hours

ARTICLE HIGHLIGHTS

Research background

In the clinic, neonatal necrotizing enterocolitis (NEC) is a frequently occurring pediatric intestinal inflammatory disease. The mortality of premature infants with NEC is as high as 50%, and the incidence shows an increasing trend, which has serious impacts on the quality of life of children.

Research motivation

This study provides help for the clinical diagnosis and treatment of neonatal NEC.

Research objectives

This study aimed to investigate the expression and significance of mucin 1 (MUC1) and interleukin-11 (IL-11) in the intestinal mucosa of children with neonatal NEC after surgery.

Research methods

Total 48 postoperative intestinal mucosal specimens from children with NEC (NEC group) and 22 intestinal mucosal specimens from congenital intestinal atresia (control group) were collected in our hospital. Immunohistochemical staining and Western-blot technique were used to detect the expression of MUC-1 protein and IL-11 protein in the two groups. The serum levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the two groups were detected by enzyme-linked immunosorbent assay, and the relationship between MUC-1 protein, IL-11 protein and serum TNF-α and IL-1β was analyzed by linear correlation method.

Research results

The protein expression of MUC-1 and IL-11 in the NEC group was significantly lower than that in the control group, and the difference was statistically significant. The levels of serum TNF-α and IL-1β in the NEC group were significantly higher than those in the control group. The protein expression of MUC-1 and IL-11 in the NEC group negatively correlated with serum TNF-α and IL-1β levels. There was a significant negative correlation between the protein expression of MUC-1 and IL-11 and the levels of serum TNF-α and IL-1β in the NEC group.

Research conclusions

The protein expression of MUC1 and IL-11 in the intestinal mucosa of children with NEC is significantly downregulated after surgery. This down regulation may be involved in the pathogenesis of the disease and has a certain correlation with inflammatory response factors in children with NEC.

Research perspectives

MUC1 and IL-11 proteins may be related to the pathogenesis of the disease and have a certain correlation with inflammatory response factors in children with NEC, which has profound clinical significance.