Clinical and Translational Research
Copyright ©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Mar 6, 2023; 11(7): 1467-1476
Published online Mar 6, 2023. doi: 10.12998/wjcc.v11.i7.1467
Analysis of differentially expressed genes related to cerebral ischaemia in young rats based on the Gene Expression Omnibus database
Yu Xia, Han Liu, Rui Zhu
Yu Xia, Rui Zhu, Department of Neurology, The Third People’s Hospital of Hefei (The Third Clinical College of Anhui Medical University), Hefei 230022, Anhui Province, China
Han Liu, Department of Neurology, The First Affiliated Hospital of Anhui Medical University (Anhui Public Health Clinical Center), Hefei 230022, Anhui Province, China
Author contributions: Xia Y and Zhu R designed the research and wrote the paper; Xia Y and Liu H performed the research; Liu H contributed new reagents; Xia Y analyzed the data.
Conflict-of-interest statement: We declare that that there is no conflict of interest to disclose.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Rui Zhu, MD, Chief Physician, Department of Neurology, The Third People’s Hospital of Hefei (The Third Clinical College of Anhui Medical University), No. 204 Wangjiang East Road, Hefei 230022, Anhui Province, China. masterzr117@sina.com
Received: December 7, 2022
Peer-review started: December 7, 2022
First decision: January 3, 2023
Revised: January 4, 2023
Accepted: February 15, 2023
Article in press: February 15, 2023
Published online: March 6, 2023
Processing time: 85 Days and 1.9 Hours
Abstract
BACKGROUND

The incidence rate of cerebral infarction in young people is increasing day by day, the age of onset tends to be younger, and its internal pathogenesis and mechanism are very complicated, which leads to greater difficulties in treatment. Therefore, it is essential to analyze the key pathway that affects the onset of cerebral infarction in young people from the perspective of genetics.

AIM

To compare the differentially expressed genes in the brain tissue of young and aged rats with middle cerebral artery occlusion and to analyse their effect on the key signalling pathway involved in the development of cerebral ischaemia in young rats.

METHODS

The Gene Expression Omnibus 2R online analysis tool was used to analyse the differentially expressed genes in the GSE166162 dataset regarding the development of cerebral ischaemia in young and aged groups of rats. DAVID 6.8 software was further used to filter the differentially expressed genes. These genes were subjected to Gene Ontology (GO) function analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis to determine the key gene pathway that affects the occurrence of cerebral ischaemia in young rats.

RESULTS

Thirty-five differentially expressed genes (such as Igf2, Col1a2, and Sfrp1) were obtained; 73 GO enrichment analysis pathways are mainly involved in biological processes such as drug response, amino acid stimulation response, blood vessel development, various signalling pathways, and enzyme regulation. They are involved in molecular functions such as drug binding, protein binding, dopamine binding, metal ion binding, and dopamine neurotransmitter receptor activity. KEGG pathway enrichment analysis showed a significantly enriched pathway: The cyclic adenosine monophosphate (c-AMP) signalling pathway.

CONCLUSION

The c-AMP signalling pathway might be the key pathway in the intervention of cerebral infarction in young people.

Keywords: Gene Expression Omnibus database; Cerebral infarction in young people; Rats; Differential gene enrichment analysis; Pathway

Core Tip: This study mainly used the analysis technology related to biogenetic informatics to retrieve the samples of young cerebral ischemia rats from the authoritative global Gene Expression Omnibus database, identified the differential genes related to the onset of cerebral ischemia in young rats through the differential gene analysis method, further carried out Gene Ontology function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes function enrichment analysis, and finally obtained the key gene pathway that affects ischaemic stroke in young rats. The findings provide clues for further realizing the transformation of basic medicine into clinical practical application, so as to finally realize the precise target intervention of young people with ischemic stroke and reduce the disability rate and mortality rate.