Basic Study
Copyright ©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Methodol. Dec 20, 2023; 13(5): 492-501
Published online Dec 20, 2023. doi: 10.5662/wjm.v13.i5.492
Urine exosome mRNA-based test for monitoring kidney allograft rejection: Effects of sample transportation and storage, and interference substances
Matt McFaul, Chris Ventura, Sean Evans, Halil Dundar, Marc J Rumpler, Christopher McCloskey, Dave Lowe, Alexandre V Vlassov
Matt McFaul, Chris Ventura, Sean Evans, Halil Dundar, Marc J Rumpler, Christopher McCloskey, Dave Lowe, Alexandre V Vlassov, Department of Research and Development, Thermo Fisher Scientific, West Hills, CA 91304, United States
Author contributions: McFaul M, Ventura C, Evans S, Dundar H performed the experiments; Rumpler MJ, McCloskey C, Lowe D, Vlassov AV planned the study and wrote the manuscript.
Institutional review board statement: The study was reviewed and approved by Thermo Fisher Scientific.
Conflict-of-interest statement: Authors are employees of Thermo Fisher Scientific.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Alexandre V Vlassov, PhD, Director, Department of Research and Development, Thermo Fisher Scientific, 22801 Roscoe Blvd, West Hills, CA 91304, United States. sasha.vlassov@thermofisher.com
Received: July 21, 2023
Peer-review started: July 21, 2023
First decision: August 31, 2023
Revised: September 7, 2023
Accepted: October 23, 2023
Article in press: October 23, 2023
Published online: December 20, 2023
Processing time: 151 Days and 21.2 Hours
Core Tip

Core Tip: Recently, it was demonstrated that analysis of urine-derived exosomal mRNA allows early detection of kidney allograft rejection. We further studied exosomes and their mRNA content as a highly promising diagnostic modality. This included stability studies of urine samples and exo-mRNA upon transportation from the point of collection to centralized testing facility, short-term storage of urine at different conditions upon receipt till the point molecular assay is performed, and effects of various interfering substances on the quantitative polymerase chain reaction assay. mRNA from the urine-derived exosomes was proven to be stable across broad range of conditions and produce robust results in post-transplant monitoring assays.