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Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.
World J Psychiatry. Jul 19, 2026; 16(7): 117078
Published online Jul 19, 2026. doi: 10.5498/wjp.117078
Effect of apple phenols on anxiety/depression in a rat model of acute exacerbation of chronic obstructive pulmonary disease
Ping Feng, Ze-Xuan Ji, Hong-Yan Zhang, Kai-Yan Song, Li-Ping Chen, Hai-Hong Qian, Bu Wang
Ping Feng, Ze-Xuan Ji, Kai-Yan Song, Li-Ping Chen, Hai-Hong Qian, Bu Wang, Respiratory and Critical Care Medicine, The First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei Province, China
Hong-Yan Zhang, Neurology III (Specialized in Psychiatry and Psychology), The First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei Province, China
Author contributions: Feng P and Ji ZX designed the research study; Zhang HY and Song KY collected and analyzed the data; Feng P and Chen LP have been involved in drafting the manuscript; Feng P, Ji ZX, Zhang HY, Song KY, Chen LP, Qian HH, and Wang B performed the research; and all authors thoroughly reviewed and endorsed the final manuscript.
Supported by Zhangjiakou Science and Technology Bureau, No. 2322090D.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the First Affiliated Hospital of Hebei North University, approval No. EARIA20230711).
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: No additional data are available.
Corresponding author: Bu Wang, Respiratory and Critical Care Medicine, The First Affiliated Hospital of Hebei North University, No. 12 Changqing Road, Zhangjiakou 075000, Hebei Province, China. fp202508@163.com
Received: January 9, 2026
Revised: February 9, 2026
Accepted: April 10, 2026
Published online: July 19, 2026
Processing time: 169 Days and 13.2 Hours
Abstract
BACKGROUND

Chronic obstructive pulmonary disease is a lung disease characterized by airflow limitation and incomplete reversibility. Apple polyphenols (AP) are secondary compounds widely present in apple metabolites. Although AP has a protective effect against acute lung injury, its effects on inflammatory factors, anxiety, and depression in rat models of acute exacerbation of chronic obstructive pulmonary disease (AECOPD) have not been studied.

AIM

To investigate the effects of AP on inflammatory factor levels and anxiety/depression in a rat model of AECOPD.

METHODS

AECOPD rat models were established using cigarette smoke inhalation and airway lipopolysaccharide injection. Seventy-two rats were divided into 6 groups: Control; model; low-dose AP (AP-L); middle-dose AP (AP-M); high-dose AP (AP-H); and AP-H + mcc950 [NOD-like receptor protein 3 (NLRP3) inhibitor]. Body weight, pulmonary function, pathological changes, and serum, lung, and prefrontal lobe levels of interleukin (IL)-6, tumor necrosis factor-α (TNF-α), IL-1β, miR-21, malondialdehyde (MDA), and lactate dehydrogenase (LDH) were assessed. Anxiety/depression were assessed using sucrose-preference and open-field experiments, and NLRP3 expression through western blotting.

RESULTS

Body weight, peak expiratory flow, peak inspiratory flow, forced expiratory volume in 0.3 seconds to forced vital capacity ratio, sucrose preference, total distance traveled, entries into and duration of stay in the central area in model group rats were reduced, while IL-6, TNF-α, IL-1β, miR-21, MDA, and LDH levels, and NLRP3 expression increased. Body weight, peak expiratory flow, peak inspiratory flow, forced expiratory volume in 0.3 seconds to forced vital capacity ratio, sucrose preference, total distance, entries into and duration of stay in the central area in the AP-L, AP-M, AP-H and AP-H + mcc950 groups increased, while NLRP3 IL-6, TNF-α, IL-1β, miR-21, MDA, and LDH levels, and NLRP3 expression were reduced. The efficacy of AP-H was superior to that of AP-M and AP-L, and mcc950 enhanced the protective effect of AP-H.

CONCLUSION

AP effectively improves body weight and lung function, reduces inflammatory cytokine and anxiety/depression levels, and alleviates pathological damage in rats with AECOPD, and is associated with downregulation of NLRP3 expression.

Keywords: Apple polyphenols; Acute exacerbation chronic obstructive pulmonary disease; Inflammatory; Anxiety-depression; NOD-like receptor protein 3

Core Tip: This study investigated the effects of apple polyphenols (AP) in a rat model of acute exacerbation of chronic obstructive pulmonary disease. Results demonstrated that AP treatment, particularly at high doses, effectively improved lung function and body weight and reduced pathological damage. It significantly downregulated the expression of NOD-like receptor protein 3 in lung tissue, leading to decreased levels of pro-inflammatory cytokines and oxidative stress markers in the serum, lung tissue, and prefrontal lobe tissue. Concurrently, AP alleviated anxiety and depression-like behaviors in acute exacerbation of chronic obstructive pulmonary disease model animals. This mechanism is associated with NOD-like receptor protein 3 pathway suppression.

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