Proteomics-based investigation of the protective effect and mechanism of Agari-5 in rats with myocardial infarction

doi:10.4330/wjc.v17.i12.112062

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Dec 26, 2025 (publication date) through Dec 24, 2025

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World Journal of Cardiology

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1949-8462

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Cardiol. Dec 26, 2025; 17(12): 112062
Published online Dec 26, 2025. doi: 10.4330/wjc.v17.i12.112062

Proteomics-based investigation of the protective effect and mechanism of Agari-5 in rats with myocardial infarction

Yu-Bao Zhao, Zhi-Hong Bao, Ya Tu, Xia Qiu, Yin-Lan Bao, Min Su, Hai-Jun Qi, Quan Wan

Yu-Bao Zhao, Zhi-Hong Bao, Ya Tu, Xia Qiu, Yin-Lan Bao, Min Su, Hai-Jun Qi, Horqin Right Wing Central Banner Mongolian Medicine Hospital, XinganLeague 137400, Inner Mongolia Autonomous Region, China

Quan Wan, Department of Cardiovascular Medicine, Affiliated Hospital of Inner Mongolia Minzu University, TongLiao 028000, Inner Mongolia Autonomous Region, China

Co-corresponding authors: Hai-Jun Qi and Quan Wan.

Author contributions: Zhao YB conducted the formal analysis and writing of the original draft; Bao ZH and Tu Y contributed to validation and methodology; Qiu X, Bao YL, and Su M conducted the formal analysis and data curation; Qi HJ and Wan Q conducted the data curation, formal analysis, investigation, methodology, and writing, reviewing, and editing as the co-corresponding authors; All authors read and approved the final version of the manuscript to be published.

Supported by XinganLeague Science and Technology Programme Project, No. MBJH2023022.

Institutional animal care and use committee statement: Animal experiments were conducted in compliance with the guidelines set by the Experimental Animal Ethics Committee of the Affiliated Hospital of Inner Mongolia Minzu University, No. NM-LL-2024-05-16-08.

Conflict-of-interest statement: The study was carried out without any financial or commercial ties that can be seen as a possible conflict of interest, according to the authors.

ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.

Data sharing statement: All data supporting the findings of this study are available within the paper and its supplementary information.

Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/

Corresponding author: Quan Wan, PhD, Associate Chief Physician, Department of Cardiovascular Medicine, Affiliated Hospital of Inner Mongolia Minzu University, No. 1742 East Section of Huolinghe Street, TongLiao 028000, Inner Mongolia Autonomous Region, China. wanquan120@126.com

Received: July 18, 2025
Revised: August 17, 2025
Accepted: October 28, 2025
Published online: December 26, 2025
Processing time: 160 Days and 3.4 Hours

Abstract

BACKGROUND

Myocardial infarction (MI) occupies a very high mortality and morbidity rate, and the search for effective pharmacological treatments has far-reaching implications for clinical research.

AIM

To explore the protective effects of Mongolian medicine Agari-5 on rats with MI.

METHODS

Sprague-Dawley rats were used, and both the Agari-5 and model groups had their coronary arteries clamped to induce MI. Proteomics was used to research the potential mechanism of action while ELISA, hematoxylin and eosin, and Masson’s staining were used to preliminarily investigate the protective impact of Agari-5 on rats with MI.

RESULTS

The current study has shown that Agari-5 might enhance cardiac function indicators, including echocardiography results of rats and creatine kinase, creatine kinase isoenzyme, and lactate dehydrogenase, in rats that had MI. According to the results of pathological staining, Agari-5 may lessen inflammatory cell infiltration and cardiomyocyte fibrosis, among other things. The proteome analysis revealed that there were 60 distinct proteins in total, four of which were associated with the heart. The expression of PSAT1, PDK1, SMAD4, and SDF2 proteins may be linked to the mechanism of their protective effects.

CONCLUSION

Potential therapeutic effects of Agari-5 for MI and its mechanism of action may be related to PSAT1, PDK1, SMAD4, and SDF2.

Keywords: Myocardial infarction; Agari-5; Proteomics; Protective effect; Mechanism

Core Tip: We demonstrated that myocardial infarction (MI) rats treated with Agari-5 expressed similar levels of SDF2, PDK1, SMAD4, and PSAT1 as the control group, suggesting that these proteins be important targets influenced by Agari-5. Through proteomic analysis the research delved deeper into the protective mechanisms of Mongolian traditional medicine formulas, finding that these traditional formulas may protect MI rats through the regulation of multiple protein targets and molecular mechanisms, such as multicomponent interactions and synergistic effects, providing a new direction for research on national medicines and offering a reference for clinical treatments of cardiovascular diseases.