Analysis of gut microbiota and serum I-FABP and PRDX1 levels in children with enterocolitis after congenital megacolon surgery

Table 1 Comparison of general data between Hirschsprung-associated enterocolitis and non-Hirschsprung-associated enterocolitis groups

	Non HAEC group (n = 225)	HAEC group (n = 109)	t/χ2	P value
Gender			0.490	0.484
Male	137 (60.89)	62 (56.88)
Female	88 (39.1)	47 (43.12)
Age (years)	2.04 ± 0.58	1.96 ± 0.57	1.127	0.261
BMI (kg/m2)	16.62 ± 1.67	16.38 ± 1.81	1.812	0.085
Spasmodic segment lesion length (cm)	17.69 ± 4.77	25.82 ± 8.74	-10.982	< 0.001
Pathological classification			1.495	0.683
Short segment type	91 (40.44)	42 (38.53)
Common types	95 (42.22)	45 (41.28)
Long segment type	23 (10.22)	10 (9.17)
Whole segment type	16 (7.11)	12 (11.02)
History of enteritis			7.263	0.007
Yes	75 (33.33)	53 (48.62)
No	150 (66.67)	56 (51.38)
Postoperative ileus (cases)			-	0.0121
Yes	4 (1.78)	9 (8.26)
No	221 (98.22)	100 (91.74)
Postoperative wound infection (cases)			-	0.0631
Yes	3 (1.33)	6 (5.5)
No	222 (98.67)	103 (94.50)

¹Use Fisher's exact probability test.

Data were showed as mean ± SD or n (%). HAEC: Hirschsprung-associated enterocolitis; BMI: Body mass index.

Table 2 Comparison of preoperative serum indexes between Hirschsprung-associated enterocolitis group and non-Hirschsprung-associated enterocolitis group

Items	Non HAEC group (n = 225)	HAEC group (n = 109)	t value	P value
CRP (mg/L)	3.58 ± 0.88	4.63 ± 1.29	-8.742	< 0.001
TNF-α (pg/mL)	32.76 ± 5.49	48.50 ± 5.76	-24.183	< 0.001
IL-6 (pg/mL)	26.00 ± 3.54	33.26 ± 5.24	-14.910	< 0.001
IL-8 (pg/mL)	16.33 ± 3.63	24.98 ± 5.04	-17.875	< 0.001
I-FABP (pg/mL)	84.35 ± 30.14	113.16 ± 35.10	-7.756	< 0.001
PRDX1 (μg/mL)	22.91 ± 6.40	32.05 ± 5.94	-12.523	< 0.001

PRDX1: Peroxiredoxin 1; I-FABP: Intestinal fatty acid-binding protein; CRP: C-reactive protein; TNF-α: Tumor necrosis factor-alpha; IL-6: Interleukin-6; IL-8: Interleukin-8.

Table 3 Logistic regression analysis results of related factors of Hirschsprung-associated enterocolitis in children with Hirschsprung’s disease after operation

	β	SE	Wals	P value	OR (95%CI)
Postoperative ileus	0.038	0.364	0.011	0.916	1.039 (0.509-2.120)
Tenericutes abundance	-0.672	0.567	1.405	0.236	0.511 (0.168-1.552)
Verrucomicrobia abundance	0.492	0.157	9.820	0.002	1.636 (1.204-2.224)
Bifidobacterium abundance	-0.003	0.001	4.379	0.036	0.997 (0.995-1.000)
Streptococcus abundance	0.004	0.003	1.778	0.250	1.004 (0.997-1.011)
Bacteroides abundance	0.010	0.003	11.111	0.003	1.010 (1.003-1.016)
CRP	0.961	0.235	16.723	< 0.001	2.614 (1.648-4.146)
I-FABP	0.031	0.007	19.621	< 0.001	1.032 (1.018-1.045)
PRDX1	0.279	0.041	46.306	< 0.001	1.322 (1.219-1.433)

PRDX1: Peroxiredoxin 1; I-FABP: Intestinal fatty acid-binding protein; CRP: C-reactive protein.