Yamamoto R, Suzuki S, Homma K, Yamaguchi S, Sujino T, Sasaki J. Hydrogen gas and preservation of intestinal stem cells in mesenteric ischemia and reperfusion. World J Gastrointest Surg 2022; 14(12): 1329-1339 [PMID: 36632117 DOI: 10.4240/wjgs.v14.i12.1329]
Corresponding Author of This Article
Koichiro Homma, MD, PhD, Assistant Professor, Department of Emergency and Critical Care Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 1608582, Japan. homma@keio.jp
Research Domain of This Article
Critical Care Medicine
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Ryo Yamamoto, Sayuri Suzuki, Koichiro Homma, Junichi Sasaki, Department of Emergency and Critical Care Medicine, Keio University School of Medicine, Tokyo 1608582, Japan
Shintaro Yamaguchi, Division of Endocrinology, Metabolism and Nephrology, Department of Internal Medicine, Keio University School of Medicine, Tokyo 1608582, Japan
Tomohisa Sujino, Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo 1608582, Japan
Author contributions: Yamamoto R, Suzuki S, Homma K, Yamaguchi S, and Sujino T designed the experiment; Yamamoto R, Suzuki S, and Homma K performed the experiment and analyzed the data; Sasaki J supervised the experiment; Yamamoto R and Suzuki S wrote the original manuscript; Yamamoto R, Suzuki S, and Homma K revised the manuscript; all authors reviewed the manuscript.
Institutional animal care and use committee statement: The protocol used in this study was approved by the Research Council and Animal Care and Use Committee of the Research Institute of Keio University in Tokyo, Japan (approval number 21013-0) and was performed in accordance with the guidelines for the care and use of laboratory animals established by the Japanese Pharmacological Society and the National Institutes of Health.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at homma@keio.jp.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Koichiro Homma, MD, PhD, Assistant Professor, Department of Emergency and Critical Care Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 1608582, Japan. homma@keio.jp
Received: August 25, 2022 Peer-review started: August 25, 2022 First decision: September 2, 2022 Revised: September 11, 2022 Accepted: November 7, 2022 Article in press: November 7, 2022 Published online: December 27, 2022 Processing time: 124 Days and 2 Hours
Core Tip
Core Tip: Distal ileum of rats was observed after 60-min mesenteric artery occlusion (ischemia), 60-min reperfusion following 60-min occlusion (reperfusion), or ischemia-reperfusion with the same duration under continuous 3% hydrogen gas inhalation (hydrogen). Immunofluorescence staining with caspase-3 and leucine-rich repeat-containing G-protein-coupled 5 (LGR5) (a specific marker of intestinal stem cell) identified ischemia damaged the epithelial layer at the tip of the villi, whereas reperfusion induced extensive apoptosis of intestinal stem cells that was mitigated by hydrogen. In addition, quantitative polymerase chain reaction revealed the LGR5 expression of the tissues was higher in rats with hydrogen inhalation than in those with reperfusion injury.
