Retrospective Study
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Diabetes. Jan 15, 2022; 13(1): 54-64
Published online Jan 15, 2022. doi: 10.4239/wjd.v13.i1.54
Role of nutritional ketosis in the improvement of metabolic parameters following bariatric surgery
Fioralba Pindozzi, Carlo Socci, Massimiliano Bissolati, Monica Marchi, Elisabetta Devecchi, Alessandro Saibene, Caterina Conte
Fioralba Pindozzi, Unità di Chirurgia Generale Provinciale, Ospedale del Delta, Lagosanto (FE) 44023, Italy
Carlo Socci, Massimiliano Bissolati, Transplant and Metabolic/Bariatric Surgery Unit, IRCCS Ospedale San Raffaele, Milan 20132, Italy
Monica Marchi, Elisabetta Devecchi, Alessandro Saibene, Department of General Medicine, Diabetes and Endocrinology, IRCCS Ospedale San Raffaele, Milan 20132, Italy
Caterina Conte, Department of Human Sciences and Promotion of the Quality of Life, San Raffaele Open University, Rome 00166, Italy
Caterina Conte, Department of Endocrinology, Nutrition and Metabolic Diseases, IRCCS MultiMedica, Milan 20099, Italy
Author contributions: Pindozzi F and Socci C contributed equally to this work; Pindozzi F and Socci C contributed to data acquisition, interpretation of data and drafting the work; Bissolati M contributed to data acquisition, Marchi M contributed to data acquisition, Devecchi E contributed to data acquisition; Saibene A contributed to data acquisition and interpretation of data; Conte C designed the work, analyzed and interpreted data; all authors have revised the work critically for important intellectual content and approve the final version of the manuscript.
Institutional review board statement: The protocol was approved by the Institutional Ethics Committee (Keto-BMS study).
Informed consent statement: All study participants, or their legal guardian, provided informed written consent prior to study enrollment.
Conflict-of-interest statement: The authors have none to declare.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Caterina Conte, MD, PhD, Associate Professor, Department of Human Sciences and Promotion of the Quality of Life, San Raffaele Open University, Via di val Cannuta, 247, Rome 00166, Italy. caterina.conte@uniroma5.it
Received: May 8, 2021
Peer-review started: May 8, 2021
First decision: June 16, 2021
Revised: June 29, 2021
Accepted: January 5, 2022
Article in press: January 5, 2022
Published online: January 15, 2022
Processing time: 247 Days and 16.2 Hours
ARTICLE HIGHLIGHTS
Research background

Ketone bodies (KB) derived from free fatty acid (FFA) metabolism serve as energy substrates in conditions of reduced glucose availability, but also as metabolic regulators and signalling molecules. Bariatric metabolic surgery (BMS) involves a marked energy deficit that results in massive mobilization of FFAs from adipose tissue, resulting in the activation of ketogenesis. It is not known whether all subjects undergoing BMS become ketotic, and whether there is a relationship between ketogenic capacity and weight loss (WL) following BMS.

Research motivation

We hypothesized that subjects with reduced ketogenic capacity are poorer responders to BMS in terms of WL. Characterization of the relationship between ketogenic capacity and WL following BMS will help understand the metabolic actions of KB and find out whether KB could be used as a predictor of BMS-induced WL.

Research objectives

We assessed the relationship between KB production in the first weeks after BMS and WL at 6 mo. We also assessed the relationship of KB with metabolic parameters and WL at 12 mo.

Research methods

For this retrospective study, we analyzed data from 39 patients who underwent laparoscopic sleeve gastrectomy, had urinary KB measured within two months of surgery and a follow-up of at least 6 mo. KB production was assessed by the presence of acetoacetic acid in urine using an automated dipstick urinalysis. We compared patients who developed post-operative ketosis with those who did not. The relationship of WL at 6 mo with pre-operative anthropometrics, body composition and metabolic parameters, and with post-operative urinary KBs was studied using bivariate correlation analyses. Variables that were significantly correlated were included in a hierarchical multiple-regression analysis, while controlling for sex, age and BMI.

Research results

This was the first study to specifically assess the relationship of ketogenic capacity with weight and metabolic outcomes. Most, but not all patients (69.2%), developed ketosis after a mean of 46.0 ± 13.6 d from surgery. Patients with ketosis were significantly younger [42.9 (37.6; 50.7) years vs 51.9 (48.3; 59.9) years, P = 0.018] and had significantly lower pre-operative fasting plasma glucose [89.5 (82.5; 96.3) mg/dL vs 96.0 (91.0; 105.3) mg/dL, P = 0.025] and triglyceride levels [108.0 (84.5; 152.5) mg/dL vs 152.0 (124.0; 186.0) mg/dL, P = 0.020], but greater LDL cholesterol (121.0 ± 23.5 mg/dL vs 100.2 ± 33.9 mg/dL, P = 0.045). WL at 6 mo was significantly greater in patients who had developed post-operative ketosis (27.5% ± 5.1% vs 23.8% ± 4.3% in the groups with and without ketosis, respectively; P = 0.035). At hierarchical multiple regression, urinary KBs and male sex emerged as significant predictors of WL at 6 mo.

Research conclusions

In keeping with the growing body of evidence indicating that nutritional ketosis has several health benefits, our findings suggest that patients who develop nutritional ketosis following BMS might have greater WL and better metabolic responses to BMS.

Research perspectives

Our findings should be considered hypothesis-generating. Further research is needed to confirm these data in larger populations, and to assess the relationship between ketogenic capacity and metabolic responses to BMS with more sophisticated techniques.