Molecular mechanism of modified Yigong San formula against colorectal cancer via EZH2/METTL3/SOX4 pathway-mediated apoptosis

Table 1 Gene primer sequences

Gene name		Primer sequence (5’-3’)
EZH2	F	5’-AATCAGAGTACATGCGACTGAGA-3’
	R	5’-GCTGTATCCTTCGCTGTTTCC-3’
METTL3	F	5’-TTGTCTCCAACCTTCCGTAGT-3’
	R	5’-CCAGATCAGAGAGGTGGTGTAG-3’
SOX4	F	5’-AGCGACAAGATCCCTTTCATTC-3’
	R	5’-CGTTGCCGGACTTCACCTT-3’
Bcl2	F	5’-GGTGGGGTCATGTGTGTGG-3’
	R	5’-CGGTTCAGGTACTCAGTCATCC-3’
Bax	F	5’-CCCGAGAGGTCTTTTTCCGAG-3’
	R	5’-CCAGCCCATGATGGTTCTGAT-3’
GAPDH	F	5’-ACAACTTTGGTATCGTGGAAGG-3’
	R	5’-GCCATCACGCCACAGTTTC-3’

EZH2: Enhancer of zeste homolog 2; METTL3: Methyltransferase-like 3; SOX4: SRY-box transcription factor 4; Bcl2: B-cell lymphoma 2.

Table 2 Chromatin immunoprecipitation-quantitative polymerase chain reaction primer sequences

Primer name		Primer sequence
METTL3 primer 1	F	AGTCACCAACATGGCAACCA
	G	AGAGCCACGTTAGGTGTTGG
METTL3 primer 2	F	ATTAACTGGGCATGGTGGCA
	G	CCTGGCCTGACACAGAGTTT

METTL3: Methyltransferase-like 3.