Fine-needle aspiration technique under endoscopic ultrasound guidance: A technical approach for RNA profiling of pancreatic neoplasms

Table 1 Indicators of quality and quantity of RNA isolated from the endoscopic ultrasound-guided fine-needle aspiration samples

EUS-FNA samples			Total, n	Lesion diameter, mm	RNA indicators
					RIN	Concentration, ng/μL	A260/A230 ratio	A260/A280 ratio
Ductal adenocarcinoma			15	45	3.9	29	1.1	1.6
				32	0	156	1.3	1.8
				39	5	618	1.9	1.9
				42	0	387	1.8	1.8
				35	7	248	2.1	1.9
				24	7	895	2.2	1.95
				38	6.9	1378	2.2	1.99
				35	7	227	1.94	1.83
				35	6.3	780	2.24	1.98
				40	5.5	1081	1.91	1.92
				38	5.1	360	1.82	1.93
				40	6.2	2044	2.13	1.98
				50	5.7	432	2.23	1.96
				40	7.8	912	2.2	1.96
				32	7.6	374	1.62	1.86
			Median (range)	38 (24-50)	6.2 (0-7.8)	432 (29-2044)	1.9 (1.1-2.2)	1.92 (1.6-2.0)
Chronic pancreatitis			5	Size not estimated	5.8	109	2.15	1.72
				-	5.1	294	2.3	1.88
				-	2.5	543	2.26	1.84
				-	6.4	68	1.9	1.84
				-	7.8	71	2.4	1.69
			Median (range)	-	5.8 (2.5-7.8)	109 (68-543)	2.3 (1.9-2.4)	1.8 (1.7-1.9)
Cysts	Fluid	MCN, IPMN	4	22	5	304	1.94	1.95
				30	7.5	201	2.21	1.86
				27	8.2	203	1.48	1.87
				19	7.9	170	2.05	1.87
			Median (range)	24.5 (19-30)	7.7 (5.0-8.2)	202 (170-304)	1.9 (1.5-2.2)	1.9 (1.9-2.0)
		SCN	4	70	0	21	0.9	1.75
				30	0	0	0	0
				57	0	0	0	0
				24	0	0	0	0
			Median (range)	44 (24-70)	0	0 (0-21)	0 (0-0.9)	0 (0-1.8)
		Pseudocyst	2	21	6.5	33.6	1.87	1.73
				35	3.5	1000	1.6	1.8
			Median (range)	28 (21-35)	5 (3.5-6.5)	516.8 (33.6-1000)	1.7 (1.6-1.9)	1.7 (1.7-1.8)
	TTNB	MCN, IPMN	3	20	0	33	1.3	2.0
				45	0	0	1.9	1.9
				38	0	23	0.27	1.9
			Median (range)	34 (20-45)	0	11.5 (0-33)	0.8 (0-1.9)	1.9 (0-1.97)
		SCN	1	30	0	0	0	0

EUS-FNA: Endoscopic ultrasound-guided, fine-needle aspiration; RIN: RNA integrity number equivalent; MCN: Mucinous cystic neoplasia; SCN: Serous cystic neoplasms; IPMN: Intraductal papillary mucinous neoplasia; TTNB: Through-the-needle biopsy.