Basic Study
Copyright ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Jun 15, 2024; 16(6): 2663-2672
Published online Jun 15, 2024. doi: 10.4251/wjgo.v16.i6.2663
Fine-needle aspiration technique under endoscopic ultrasound guidance: A technical approach for RNA profiling of pancreatic neoplasms
Sabina Sherafedinovna Seyfedinova, Olga Aleksandrovna Freylikhman, Polina Sergeevna Sokolnikova, Konstantin Aleksandrovich Samochernykh, Anna Aleksandrovna Kostareva, Olga Viktorovna Kalinina, Evgeniy Gennadievich Solonitsyn
Sabina Sherafedinovna Seyfedinova, Olga Aleksandrovna Freylikhman, Evgeniy Gennadievich Solonitsyn, Research Laboratory of Digestive Cancer, Almazov Medical Research Centre, Saint-Petersburg 197341, Russia
Polina Sergeevna Sokolnikova, Research Laboratory of Molecular and Cellular Modeling and Gene Therapy, Almazov Medical Research Centre, Saint-Petersburg 197341, Russia
Konstantin Aleksandrovich Samochernykh, Head of Russian Neurosurgical Institute Named after Prof. A. L. Polenova, Russian Neurosurgical Institute Named after Prof. A. L. Polenova, Saint-Petersburg 191014, Russia
Anna Aleksandrovna Kostareva, Head of Institute of Molecular Biology and Genetics, Almazov National Medical Research Centre, Saint-Petersburg 197341, Russia
Olga Viktorovna Kalinina, Research Laboratory of Autoimmune and Autoinflammatory Diseases, Almazov National Medical Research Centre, Saint-Petersburg 197341, Russia
Co-first authors: Sabina Sherafedinovna Seyfedinova and Olga Aleksandrovna Freylikhman.
Author contributions: Freylikhman OA as a specialist in molecular biology conducted technical part of the investigation and descripted methods and results in the article, contributed in the methodology development. Seyfedinova SS and Freylikhman OA are co-first authors of this manuscript. Seyfedinova SS, Freylikhman OA, and Sokolnikova PS contributed to the data curation and investigation; Seyfedinova SS, Freylikhman OA, Samochernykh KA, Kostareva AA, Kalinina OV, and Solonitsyn EG were involved in the methodology of this study; Seyfedinova SS and Freylikhman OA participated in the writing-original draft; Samochernykh KA and Kostareva AA contributed to the supervision of this manuscript; Kostareva AA, Kalinina OV, and Solonitsyn EG were involved in the validation and editing of this article; Kalinina OV and Solonitsyn EG contributed to the conceptualization.
Supported by the Ministry of Science and Higher Education of the Russian Federation, No. 075-15-2022-301.
Institutional review board statement: All samples were obtained for scientific research according to the Helsinki Declaration and approved by the Almazov National Medical Centre ethics committee (Approval No. 2101-23).
Informed consent statement: All study participants, or their legal guardians, provided informed written consent before study enrollment.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at seysabina000@gmail.com. No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Sabina Sherafedinovna Seyfedinova, Doctor, Research Scientist, Research Laboratory of Digestive Cancer, Almazov Medical Research Centre, Akkuratova Av. 2, Saint-Petersburg 197341, Russia. seysabina000@gmail.com
Received: December 26, 2023
Revised: February 18, 2024
Accepted: April 7, 2024
Published online: June 15, 2024
Processing time: 171 Days and 19.2 Hours
Core Tip

Core Tip: In this study, the use of RNA from endoscopic ultrasound-guided, fine-needle aspiration (EUS-FNA) samples of cystic and solid pancreatic neoplasms, including samples of low-quality RNA, was demonstrated. The process of sample preparation from EUS-FNA material is detailed along with the necessary criteria for assessing RNA quality for effective molecular biological analysis. Issues regarding the dependence of RNA quality on the type of neoplasm and material are identified. Modification of the library preparation protocol made it possible to obtain libraries for samples with RNA integrity number equivalent values of three and above, meeting the quality criteria for purity and concentration.