Human colorectal cancer cells frequently express IgG and display unique Ig repertoire

Table 1 Clinical information of 7 patients with colon cancer

ID	Sex	Age	Clinical diagnosis	Tumor size (cm)	Differentiation	Vascular invasion	LNM	Distant metastasis	TMN	Histological type	MLH1	MSH2	MSH6	PMS2
1	F	77	Horizontal colon cancer	5.2 × 4.9	Moderately and poorly differentiated	+	0	N/A	T4aN0M0	Adenocarcinoma	N/A	N/A	N/A	N/A
2	F	63	Sigmoid colon cancer	N/A	Poorly differentiated	N/A	N/A	N/A	T3aN1bM0	Adenocarcinoma	N/A	N/A	N/A	N/A
3	M	77	Right-sided colon cancer	3 × 3	Moderately differentiated	N/A	0	N/A	T4aN0M0	Adenocarcinoma	+	+	+	-
4	F	52	Right-sided colon cancer	4 × 2.5	Well differentiated	N/A	0	N/A	TisN0M0	Adenocarcinoma	+	+	+	±
5	M	61	Colon cancer	2.8 × 1.8	Moderately differentiated	+	1/12	Sacrum metastasis	T4N1M1	Adenocarcinoma	+	+	+	±
6	M	74	Right-sided colon cancer	12 × 9 × 7	N/A	+	15/17	N/A	T4N3M1	Adenocarcinoma	+	+	+	+
7	M	89	Right-sided colon cancer	8.5 × 5	Moderately differentiated	N/A	0	Small bowel metastasis	T4bN0M0	Adenocarcinoma	±	±	±	±

Table 2 Top 10 of V_HDJ_H rearrangement patterns in colon cancer and normal epithelial cells

	Cancer				Normal
	V	D	J	%	V	D	J	%
Patient-1	hIGHV3-13	hIGHD3-9	hIGHJ6	66.84%	hIGHV1-8	hIGHD7-27	hIGHJ4	27.25%
	hIGHV3-30	hIGHD6-19	hIGHJ6	15.81%	hIGHV3-74	hIGHD6-19	hIGHJ4	24.71%
	hIGHV3-30-3	hIGHD2-8	hIGHJ4	4.90%	hIGHV3-23	hIGHD2-21	hIGHJ5	18.58%
	hIGHV5-51	hIGHD3-16	hIGHJ4	3.69%	hIGHV1-8	hIGHD1-7	hIGHJ3	6.31%
	hIGHV3-23	hIGHD6-13	hIGHJ4	3.58%	hIGHV5-51	hIGHD3-16	hIGHJ4	5.00%
	hIGHV3-7	hIGHD3-9	hIGHJ6	1.00%	hIGHV3-23	hIGHD3-22	hIGHJ6	2.92%
	hIGHV3-33	hIGHD6-19	hIGHJ6	0.79%	hIGHV3-74	hIGHD4-17	hIGHJ4	2.20%
	hIGHV3-13	hIGHD6-13	hIGHJ6	0.53%	hIGHV3-21	hIGHD2-15	hIGHJ5	1.19%
	hIGHV3-30	hIGHD2-8	hIGHJ4	0.37%	hIGHV3-74	hIGHD2-21	hIGHJ5	1.17%
	hIGHV3-64	hIGHD6-19	hIGHJ6	0.26%	hIGHV3-23	hIGHD6-19	hIGHJ4	1.06%
Patient-2	hIGHV1-58	hIGHD3-22	hIGHJ4	43.86%	hIGHV1-18	hIGHD4-17	hIGHJ4	24.34%
	hIGHV1-46	hIGHD3-9	hIGHJ4	18.00%	hIGHV3-11	hIGHD5-5	hIGHJ2	10.95%
	hIGHV3-43	hIGHD3-22	hIGHJ4	10.20%	hIGHV3-74	hIGHD6-13	hIGHJ1	10.28%
	hIGHV3-23	hIGHD3-9	hIGHJ4	6.28%	hIGHV3-9	hIGHD6-19	hIGHJ4	10.02%
	hIGHV1-69	hIGHD4-17	hIGHJ3	5.31%	hIGHV3-23	hIGHD3-10	hIGHJ4	6.67%
	hIGHV1-69	hIGHD2-15	hIGHJ5	3.98%	hIGHV4-4	hIGHD3-22	hIGHJ4	5.92%
	hIGHV1-2	hIGHD5-5	hIGHJ4	2.77%	hIGHV3-15	hIGHD3-10	hIGHJ4	3.87%
	hIGHV1-58	hIGHD5-5	hIGHJ4	1.08%	hIGHV3-49	hIGHD6-19	hIGHJ4	2.88%
	hIGHV3-53	hIGHD6-6	hIGHJ4	1.01%	hIGHV4-59	hIGHD3-22	hIGHJ4	2.34%
	hIGHV1-3	hIGHD7-27	hIGHJ6	0.61%	hIGHV3-53	hIGHD6-19	hIGHJ4	2.14%
Patient-3	hIGHV3-23	hIGHD4-4	hIGHJ5	11.63%	hIGHV1-18	hIGHD4-17	hIGHJ4	24.71%
	hIGHV7-4-1	hIGHD1-26	hIGHJ6	11.28%	hIGHV3-9	hIGHD6-19	hIGHJ4	11.82%
	hIGHV3-74	hIGHD6-6	hIGHJ5	11.16%	hIGHV3-11	hIGHD5-5	hIGHJ2	10.21%
	hIGHV3-23	hIGHD3-16	hIGHJ5	6.15%	hIGHV3-74	hIGHD6-13	hIGHJ1	9.42%
	hIGHV3-48	hIGHD4-4	hIGHJ4	5.29%	hIGHV3-23	hIGHD3-10	hIGHJ4	6.20%
	hIGHV2-5	hIGHD5-12	hIGHJ4	5.16%	hIGHV4-4	hIGHD3-22	hIGHJ4	5.93%
	hIGHV1-69	hIGHD5-5	hIGHJ4	4.83%	hIGHV3-15	hIGHD3-10	hIGHJ4	4.39%
	hIGHV3-23	hIGHD3-22	hIGHJ5	4.18%	hIGHV4-59	hIGHD3-22	hIGHJ4	2.78%
	hIGHV3-53	hIGHD5-12	hIGHJ4	2.14%	hIGHV3-49	hIGHD6-19	hIGHJ4	2.50%
	hIGHV3-11	hIGHD2-15	hIGHJ4	2.07%	hIGHV3-53	hIGHD6-19	hIGHJ4	2.06%
Patient-4	hIGHV3-7	hIGHD3-22	hIGHJ5	57.78%	hIGHV3-74	hIGHD2-8	hIGHJ5	88.00%
	hIGHV3-48	hIGHD3-22	hIGHJ5	13.33%	hIGHV1-8	hIGHD7-27	hIGHJ4	4.00%
	hIGHV1-58	hIGHD3-22	hIGHJ4	11.11%	hIGHV3-74	hIGHD6-19	hIGHJ4	2.00%
	hIGHV3-43	hIGHD3-22	hIGHJ4	6.67%	hIGHV3-74	hIGHD2-21	hIGHJ5	2.00%
	hIGHV3-23	hIGHD3-9	hIGHJ4	4.44%	hIGHV3-23	hIGHD2-21	hIGHJ5	2.00%
	hIHGV3-74	hIGHD2-8	hIGHJ5	2.22%	hIGHV3-74	hIGHD5-5	hIGHJ6	2.00%
	hIGHV3-21	hIGHD3-22	hIGHJ5	2.22%	N/A
	hIHGV1-46	-	hIGHJ4	2.22%	N/A
Patient-5	hIHGV3-7	hIHGD3-22	hIHGJ5	65.96%	hIGHV3-7	hIGHD3-22	hIGHJ5	60.00%
	hIHGV3-21	hIHGD3-22	hIHGJ5	4.26%	hIGHV1-8	hIGHD7-27	hIGHJ4	12.31%
	hIHGV3-74	hIHGD6-6	hIHGJ5	4.26%	hIGHV1-8	hIGHD1-7	hIGHJ3	6.15%
	hIHGV7-4-1	hIHGD1-26	hIHGJ6	4.26%	hIGHV3-23	hIGHD2-21	hIGHJ5	4.62%
	hIHGV3-48	hIHGD3-22	hIHGJ5	4.26%	hIGHV3-74	hIGHD6-19	hIGHJ4	4.62%
	hIHGV3-48	hIHGD4-4	hIHGJ4	4.26%	hIGHV3-74	hIGHD2-8	hIGHJ4	3.08%
	hIHGV3-11	hIHGD2-15	hIHGJ4	2.13%	hIGHV5-51	hIGHD3-16	hIGHJ4	1.54%
	hIHGV3-33	hIHGD3-22	hIHGJ5	2.13%	hIGHV3-9	hIGHD2-21	hIGHJ5	1.54%
	hIHGV3-23	hIHGD3-22	hIHGJ5	2.13%	hIGHV3-33	hIGHD3-22	hIGHJ5	1.54%
	hIHGV3-72	hIHGD1-7	hIHGJ6	2.13%	hIGHV3-74	hIGHD6-25	hIGHJ4	1.54%
Patient-6	hIGHV1-8	hIGHD6-13	hIGHJ5	44.32%	N/A
	hIGHV4-61	hIGHD3-16	hIGHJ6	16.60%
	hIGHV1-8	hIGHD3-10	hIGHJ5	14.34%
	hIGHV3-23	hIGHD3-22	hIGHJ3	11.74%
	hIGHV1-8	hIGHD7-27	hIGHJ4	6.00%
	hIGHV3-23	hIGHD2-21	hIGHJ5	4.57%
	hIGHV3-74	hIGHD6-19	hIGHJ4	0.96%
	hIGHV4-61	hIGHD2-21	hIGHJ6	0.17%
	hIGHV3-74	hIGHD3-22	hIGHJ3	0.13%
	hIGHV1-8	hIGHD3-16	hIGHJ5	0.13%
Patient-7	hIGHV3-7	hIGHD3-22	hIGHJ5	80.95%	N/A
	hIGHV3-21	hIGHD3-22	hIGHJ5	1.28%
	hIGHV3-33	hIGHD3-22	hIGHJ5	0.92%
	hIGHV3-23	hIGHD4-4	hIGHJ5	0.92%
	hIGHV7-4-1	hIGHD1-26	hIGHJ6	0.92%
	hIGHV3-23	hIGHD3-22	hIGHJ5	0.73%
	hIGHV3-48	hIGHD3-22	hIGHJ5	0.73%
	hIGHV2-5	hIGHD5-12	hIGHJ4	0.73%
	hIGHV3-48	hIGHD4-4	hIGHJ4	0.55%
	hIGHV3-48	hIGHD3-22	hIGHJ5	0.55%