Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Mar 15, 2019; 11(3): 195-207
Published online Mar 15, 2019. doi: 10.4251/wjgo.v11.i3.195
Human colorectal cancer cells frequently express IgG and display unique Ig repertoire
Zi-Han Geng, Chun-Xiang Ye, Yan Huang, Hong-Peng Jiang, Ying-Jiang Ye, Shan Wang, Yuan Zhou, Zhan-Long Shen, Xiao-Yan Qiu
Zi-Han Geng, Xiao-Yan Qiu, Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, China
Zi-Han Geng, Xiao-Yan Qiu, NHC Key Laboratory of Medical Immunology (Peking University), Beijing 100191, China
Zi-Han Geng, Xiao-Yan Qiu, Key Laboratory of Molecular Immunology, Chinese Academy of Medical Sciences, Beijing 100191, China
Chun-Xiang Ye, Hong-Peng Jiang, Ying-Jiang Ye, Shan Wang, Zhan-Long Shen, Department of Gastrointestinal Surgery, Peking University People's Hospital, Beijing 100044, China
Chun-Xiang Ye, Hong-Peng Jiang, Ying-Jiang Ye, Shan Wang, Zhan-Long Shen, Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Beijing 100044, China
Yan Huang, Institute of Computational Medicine, School of Artificial Intelligence, Hebei University of Technology, Tianjin 300401, China
Yuan Zhou, Department of Biomedical Informatics, School of Basic Medical Sciences, Center for Noncoding RNA Medicine, Peking University, Beijing 100191, China
Author contributions: Qiu XY and Shen ZL initiated and designed the research; Geng ZH and Ye CX performed all the experiments; Huang Y and Zhou Y carried out data analyzing; Jiang HP contributed in analyzing and interpreting results; Geng ZH and Qiu XY wrote the manuscript; Ye YJ, Wang S contributed in clinical diagnosis of patients; Jiang HP and Shen ZL provided clinical specimens, and clinical and pathological information.
Supported by Key support projects of the National Natural Science Foundation's major research program, No. 91642206; Major international cooperation projects of the National Natural Science Foundation, No. 81320108020; Beijing Natural Science Foundation, No. 7182171; Research institute fund of NHC Key Laboratory of Medical Immunology, Peking University, No. BMU2018JDJS010; and Non-profit central research institute fund of Chinese Academy of Medical Sciences, No. 2018PT31039.
Institutional review board statement: This work is supported by Medical Ethics Committee of Peking University People's Hospital (2018PHB 193-01).
Conflict-of-interest statement: The authors have no conflicts of interest to declare.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Xiao-Yan Qiu, MD, PhD, Doctor, Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Xueyuan Road, Beijing 100191, China. qiuxy@bjmu.edu.cn
Telephone: +86-10-82805477 Fax: +86-10-82801149
Received: October 26, 2018
Peer-review started: October 26, 2018
First decision: November 14, 2018
Revised: January 3, 2019
Accepted: January 8, 2019
Article in press: January 9, 2019
Published online: March 15, 2019
Processing time: 140 Days and 19.2 Hours
Core Tip

Core tip: It has been found that colon cancer cells can express immunoglobulin (Ig); however, the expression profile and features of the Ig repertoire in colon cancer cells remain unclear. Here, we first sorted colon cancer cells and normal cells from 7 patients with colon cancer. Using the Ig repertoire sequencing, we analyzed the features of the Ig heavy chain (IgH) repertoire in these cells. We found that Ig in colon cancer cells had a significant tendency to choose IgG compared to the other classes of IgH, and showed unique VHDJH patterns and somatic hypermutation hotspots, which might be potential targets for immune therapy for colon cancer.