Meta-Analysis
Copyright ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Apr 15, 2024; 16(4): 1626-1646
Published online Apr 15, 2024. doi: 10.4251/wjgo.v16.i4.1626
Success rate of current human-derived gastric cancer organoids establishment and influencing factors: A systematic review and meta-analysis
Kai-Lin Jiang, Xiang-Xiang Wang, Xue-Jiao Liu, Li-Kun Guo, Yong-Qi Chen, Qing-Ling Jia, Ke-Ming Yang, Jiang-Hong Ling
Kai-Lin Jiang, Xiang-Xiang Wang, Xue-Jiao Liu, Li-Kun Guo, Qing-Ling Jia, Ke-Ming Yang, Jiang-Hong Ling, Department of Gastroenterology, Shuguang Hospital, Shanghai 200021, China
Yong-Qi Chen, Department of Pathology, Shuguang Hospital, Shanghai 200021, China
Co-first authors: Kai-Lin Jiang and Xiang-Xiang Wang.
Author contributions: Jiang KL and Ling JH contributed to the conceptualization; Wang XX was involved in the methodology, literature searching, and data extraction; Liu XJ and Chen YQ are responsible for the software; Jia QL and Yang KM contributed to the formal analysis; Jiang KL and Liu XJ wrote the manuscript; Jiang KL contributed to the figure and table; Ling JH participated in the funding acquisition; and all authors have read and agreed to the published version of the manuscript.
Supported by National Natural Science Foundation of China, No. 82174309 and No. 81973774; National Administration of Traditional Chinese Medicine: 2019 Project of Building Evidence-Based Practice Capacity for TCM, No. 2019XZZX-XH013; and Shuguang Hospital Siming Foundation Research Special Project, No. SGKJ-202304.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
PRISMA 2009 Checklist statement: The authors have read the PRISMA 2009 Checklist, and the manuscript was prepared and revised according to the PRISMA 2009 Checklist.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Jiang-Hong Ling, PhD, Professor, Department of Gastroenterology, Shuguang Hospital, No. 185 Pu’an Road, Huangpu District, Shanghai 200021, China. ljh18817424778@163.com
Received: October 2, 2023
Peer-review started: October 2, 2023
First decision: January 5, 2024
Revised: January 18, 2024
Accepted: February 29, 2024
Article in press: February 29, 2024
Published online: April 15, 2024
Processing time: 191 Days and 10.5 Hours
Core Tip

Core Tip: This study systematically reviewed the success rate of establishing human-derived gastric cancer organoids (GCOs), highlighting the relatively low overall success rate that is influenced by factors such as sex, tissue source, histological type, and pathological tumor-node-metastasis cancer stage. Our meta-analysis revealed that omitting the Rho Kinase inhibitor Y-27632 and using certain digestive enzymes, such as collagenase, enhanced culture success. These findings suggest potential avenues for improving GCO culture techniques that are crucial for advancing gastric cancer research and personalized medicine.