Published online Oct 15, 2020. doi: 10.4251/wjgo.v12.i10.1091
Peer-review started: March 2, 2020
First decision: April 25, 2020
Revised: May 18, 2020
Accepted: May 27, 2020
Article in press: May 27, 2020
Published online: October 15, 2020
Processing time: 226 Days and 1.6 Hours
New therapeutic agents for liver cancer, which can control inflammation and restore cellular immunity, are required. Curcumin (Cur) is a natural anti-inflammatory drug and total ginsenosides (TG) are a commonly used immunoregulatory drug.
Both Cur and TG have been shown to exert anti-liver cancer effects. This study discussed the anti-tumor effect of Cur combined with TG in liver cancer and its molecular mechanism.
To determine the synergistic immunomodulatory and anti-inflammatory effects of Cur combined with TG in a mouse model of subcutaneous liver cancer.
The changes in tumor volume and expression of relevant factors were compared in a subcutaneous liver cancer mouse model after treatment with Cur, TG, and the combination of the two drugs. The protein expression of programmed cell death 1 (PD-1) and PD-1 ligand 1 (PD-L1), inflammatory indicators Toll like receptor 4 (TLR4) and nuclear factor-κB (NF-κB), and vascular growth-related factors nitric oxide synthases (iNOS) and matrix metalloproteinase 9 were analyzed by Western blot analysis. CD4+CD25+Foxp3+ regulatory T cells (Tregs) were counted by flow cytometry.
The combination therapy of Cur and TG significantly inhibited the growth of liver cancer, and TG showed dose dependence. Cur combined with TG-520 markedly decreased the protein expression of PD-L1 (P < 0.0001), while CD4+CD25+Foxp3+ Tregs regulated by the PD-L1 signaling pathway exhibited a positive correlation with PD-L1. Cur combined with TG-520 also inhibited the cascade action mediated by NF-κB (P < 0.0001), inhibiting the TLR4/NF-κB signaling pathway (P = 0.0088, P < 0.0001), which is associated with inflammation and acts on PD-L1. It also inhibited the NF-κB-MMP9 signaling pathway (P < 0.0001), which is associated with tumor angiogenesis.
Cur combined with TG regulates immune escape through the PD-L1 pathway and inhibits liver cancer growth through NF-κB-mediated inflammation and angiogenesis.
This study offers a potential combination of drugs that could improve the effectiveness of treatment for liver cancer.