Niu W, Bo QY, Niu J, Niu ZC, Peng C, Zou XQ, Zhang ZY. Identification of integrin β6 gene promoter and analysis of its transcription regulation in colon cancer cells. World J Gastrointest Oncol 2020; 12(5): 526-534 [PMID: 32461784 DOI: 10.4251/wjgo.v12.i5.526]
Corresponding Author of This Article
Zhao-Yang Zhang, MD, Associate Chief Physician, Associate Professor, Department of Emergency Surgery, Qilu Hospital, Shandong University, No. 44, Wenhua Xi Road, Jinan 250012, Shandong Province, China.drzzyang@163.com
Research Domain of This Article
Oncology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Wei Niu, Jun Niu, Zheng-Chuan Niu, Cheng Peng, Xue-Qing Zou, Department of Hepatobiliary Surgery, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China
Qi-Yu Bo, Department of Nursing, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China
Zhao-Yang Zhang, Department of Emergency Surgery, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China
Author contributions: Niu W and Bo QY contributed equally to this article and should be considered as co-first authors; Niu W and Bo QY designed the experiments; Zhang ZY drafted the work and collected the data; Niu J, Niu ZC, Peng C, Zou XQ, and Zhang ZY analysed and interpreted the data and wrote the article.
Supported byNational Sciences Foundation of Shandong Province, No. ZR2014HM101.
Institutional review board statement: The study was reviewed and approved by the Institutional Review Board of Qilu Hospital, Shandong University.
Conflict-of-interest statement: The authors declare no conflicts of interest related to this manuscript.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Zhao-Yang Zhang, MD, Associate Chief Physician, Associate Professor, Department of Emergency Surgery, Qilu Hospital, Shandong University, No. 44, Wenhua Xi Road, Jinan 250012, Shandong Province, China.drzzyang@163.com
Received: January 20, 2020 Peer-review started: January 20, 2020 First decision: March 24, 2020 Revised: March 29, 2020 Accepted: April 15, 2020 Article in press: April 15, 2020 Published online: May 15, 2020 Processing time: 115 Days and 0.7 Hours
Abstract
BACKGROUND
The integrin β6 gene, which is expressed in epithelial cancer, plays a pivotal role in various aspects of cancer progression. The present research for integrin β6 regulation mainly focuses on the post-transcription and translation related regulation mechanism and its role in tumorigenesis. The mechanisms of how the integrin β6 gene is regulated transcriptionally, and the promoter and transcription factors responsible for basic transcription of integrin β6 gene remain unknown.
AIM
To clone and characterize the integrin β6 promoter.
METHODS
Software analysis was used to predict the region of integrin β6 promoter. Luciferase reporter plasmids, which contained the integrin β6 promoter, were constructed. Element deletion analysis was performed to identify the location of core promoter and binding sites for transcription factors.
RESULTS
The regulatory elements for the transcription of the integrin β6 gene were located between -286 and -85 and contained binding sites for transcription factors such as STAT3 and Ets-1.
CONCLUSION
For the first time, we found the region of β6 core promoter and demonstrated the binding sites for transcription factors such as Ets-1 and STAT3, which are important for integrin β6 promoter transcription activity. These findings are important for investigating the mechanism of integrin β6 activation in cancer progression.
Core tip: In this paper, the transcription regulation of integrin β6 is described and the region of core promoter region is identified. Meanwhile, the mechanism of transcription regulation of integrin β6 is explored.
