Discussion on the effect of Honghua Qinggan Shisanwei Wan on regulating macrophage polarization and alleviating liver injury

doi:10.4254/wjh.118215

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World Journal of Hepatology

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1948-5182

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.

World J Hepatol. Jun 27, 2026; 18(6): 118215
Published online Jun 27, 2026. doi: 10.4254/wjh.118215

Table 1 Primer sequences

Gene	Forward primer	Reverse primer
TNF-α	CCCTCACACTCAGATCATCTTCT	GCTACGACGTGGGCTACAG
IL-6	CCAAGAGGTGAGTGCTTCCC	CTGTTGTTCAGACTCTCTCCCT
IL-1β	GCAACTGTTCCTGAACTCAACT	ATCTTTTGGGGTCCGTCAACT
CCL2	TTAAAAACCTGGATCGGAACCAA	GCATTAGCTTCAGATTTACGGGT
F4/80	TGACTCACCTTGTGGTCCTAA	CTTCCCAGAATCCAGTCTTTCC
TGF-β	CTCCCGTGGCTTCTAGTGC	GCCTTAGTTTGGACAGGATCTG
YM-1	CAGGTCTGGCAATTCTTCTGAA	GTCTTGCTCATGTGTGTAAGTGA
IL-10	GCTCTTACTGACTGGCATGAG	CGCAGCTCTAGGAGCATGTG
CX3CR1	GAGTATGACGATTCTGCTGAGG	CAGACCGAACGTGAAGACGAG
NR4A1	TTGAGTTCGGCAAGCCTACC	GTGTACCCGTCCATGAAGGTG
NR4A2	GTGTTCAGGCGCAGTATGG	TGGCAGTAATTTCAGTGTTGGT
INOS	GTTCTCAGCCCAACAATACAAGA	GTGGACGGGTCGATGTCAC
β-actin	GGCTGTATTCCCCTCCATCG	CCAGTTGGTAACAATGCCATGT

TNF-α: Tumor necrosis factor-α; interleukin; IL: Interleukin; CCL2: C-C motif chemokine ligand 2; TGF: Transforming growth factor; CX3CR1: C-X3-C motif chemokine receptor 1; NR4A: Nuclear receptor subfamily 4 group A; iNOS: Inducible nitric oxide synthase.

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Table 2 Changes in the expression of macrophage polarization-related genes in Honghua Qinggan Shisanwei Wan

Gene name	D-5	T-5	log2 fold change	P value
Pparg	162.46	98.41	-0.7190	0.00517415
Stat1	723.84	320.51	-1.1774	6.01E-11
Csf1	355.52	200.93	-0.8192	1.19E-05
Stat2	1410.80	895.83	-0.6543	7.52E-06
Tlr2	238.08	75.68	-1.6471	1.21E-09
Nfekbie	105.56	51.49	-1.0276	0.00017910
Csf3r	219.95	117.95	-0.8960	0.00092885
Tlr4	224.86	63.67	-1.8085	1.99E-10
Irf1	709.64	265.39	-1.4161	1.09E-07
Csf1r	3924.30	1401.87	-1.4844	2.11E-12
Csf2rb	750.01	204.98	-1.8676	9.20E-12
Cd163	1221.77	394.60	-1.6296	4.51E-25
Csf2ra	246.42	104.53	-1.2365	7.50E-09
Csf2rb2	280.50	62.45	-2.1636	1.34E-16
CD86	217.67	107.85	-1.0108	1.42E-05
Arg2	23.43	8.33	-1.4792	0.00882463
Klf4	95.40	53.11	0.8507	0.00486715
Stat5b	1046.13	1806.45	0.7879	1.93E-10
Ppargc1a	167.31	376.35	1.1685	5.96E-06
Nr4A3	6.19	49.79	3.0034	2.18E-07
Cebpb	1472.50	4302.12	1.3658	3.08E-09
Ppargc1b	70.46	133.48	0.9218	0.00012760
Nr4A1	70.71	722.02	3.3517	1.15E-06
Nr4A2	6.50	31.72	2.2805	0.00035749

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Table 3 The expression of related genes in the model group and the control group

Gene name	D-5	C	log2 fold change	P value
CCR2	431.029	29.565	3.8715	4.42E-27
Ly6C1	65.660	12.382	2.4093	3.90E-09
Ly6C2	277.276	29.254	3.2445	6.86E-14

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Table 4 The expression of related genes in the model groupand the Honghua Qinggan Shisanwei Wan hepatoprotective group

Gene name	D-5	C	log2 fold change	P value
CCR2	391.564	55.161	-2.8240	1.66E-15
Ly6C1	57.467	15.409	-1.9182	3.18E-05
Ly6C2	254.435	28.409	-3.1597	4.95E-14
Cx3cr1	36.552	347.887	3.2465	4.77E-06
Fcgr4	185.408	1113.257	2.5863	1.19E-26

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Citation: Ma CL, Zhang X, Bao X, Zhao LY, Cao LL, Ma CY, Zhang T, Hong M, Bao YL, Hu RP. Discussion on the effect of Honghua Qinggan Shisanwei Wan on regulating macrophage polarization and alleviating liver injury. World J Hepatol 2026; 18(6): 118215
URL: https://www.wjgnet.com/1948-5182/full/v18/i6/118215.htm
DOI: https://dx.doi.org/10.4254/wjh.118215

Ma CL, Zhang X, Bao X, Zhao LY, Cao LL, Ma CY, Zhang T, Hong M, Bao YL, Hu RP. Discussion on the effect of Honghua Qinggan Shisanwei Wan on regulating macrophage polarization and alleviating liver injury. World J Hepatol 2026; 18(6): 118215 [DOI: 10.4254/wjh.118215]

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