Lv F, Gao YF, Zhang ZH, Zhang TC, Pan FM, Cui MF, Xia SL, Li X, Yin HF. Polymorphisms in programmed death-1 gene are not associated with chronic HBV infection in Chinese patients. World J Hepatol 2011; 3(3): 72-78 [PMID: 21487538 DOI: 10.4254/wjh.v3.i3.72]
Corresponding Author of This Article
Hua-Fa Yin, Professor, Department of Infectious Diseases, the First Affiliated Hospital, Anhui Medical University, 218 Jixi Road, Hefei 230022, Anhui Province, China. yhf163.good@163.com
Article-Type of This Article
Original Article
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Feng Lv, Yu-Feng Gao, Zhen-Huan Zhang, Ming-Fang Cui, Shu-Ling Xia, Xu Li, Hua-Fa Yin, Department of Infectious Diseases, the First Affiliated Hospital, Anhui Medical University, Hefei 230022, Anhui Province, China
Tian-Chen Zhang, Fa-Ming Pan, Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei 230032, Anhui Province, China
Author contributions: Lv F and Gao YF contributed equally to this work; Zhang ZH provided the vital reagents and analytical tools; Lv F and Gao YF wrote the manuscript; Lv F, Cui MF and Xia SL performed the majority of experiments; Zhang TC and Pan FM took charge of collecting all human materials; Yin HF designed the study; and Li X provided the financial support for this work.
Supported by the Grants from the National Natural Science Foundation of China, No.30700698 and 30771907 and the Foundation of Pre-973 Program Projects, No. 2009CB526411
Correspondence to: Hua-Fa Yin, Professor, Department of Infectious Diseases, the First Affiliated Hospital, Anhui Medical University, 218 Jixi Road, Hefei 230022, Anhui Province, China. yhf163.good@163.com
Telephone: +86-551-2922912 Fax: +86-551-2922912
Received: November 26, 2010 Revised: December 23, 2010 Accepted: December 30, 2010 Published online: March 27, 2011
Abstract
AIM: To investigate the association between the programmed death-1(PD-1) polymorphisms and genetic susceptibility of chronic hepatitis B virus (HBV) infection in Chinese patients.
METHODS: Two single nucleotide polymorphisms (SNPs), PD-1.1 G > A and PD-1.2 G > A, were genotyped in 539 patients with chronic HBV infection and 353 other family members (HbsAg-) from 256 nuclear families using polymerase chain reactiorestriction fragment length polymorphisms assay. The associations between PD-1 polymorphisms and genetic susceptibility of chronic HBV infection were analyzed usng the family-based association analysis method.
RESULTS: No association or linkage was detected among 539 patients. Univariate (single-marker) family-based association tests demonstrated that PD-1 genotypes, alleles and transmitted haplotypes are not associated with chronic HBV infection (all with P value more than 0.05). Transmission/disequilibrium test and sibship disequilibrium test analysis showed no excess of the alleles from heterozygous parents to affected offspring (P = 0.688880, P = 1.000000 respectively).
CONCLUSION: The data demonstrated that PD-1.1 and PD-1.2 polymorphisms are not associated with chronic HBV infection in Chinese patients.
