Hypoxic preconditioning restores the regenerative potential of aged adipose tissue derived mesenchymal stem cells

doi:10.4252/wjsc.v18.i5.117241

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World Journal of Stem Cells

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1948-0210

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Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.

World J Stem Cells. May 26, 2026; 18(5): 117241
Published online May 26, 2026. doi: 10.4252/wjsc.v18.i5.117241

Table 1 Primer sequences used for target gene amplification

Gene	Sequence	Annealing temperature
VEGF	TTGCCTTGCTGCTCTACCTCCA	60 °C
VEGF	GATGGCAGTAGCTGCGCTGATA	60 °C
AKT1	TGGACTACCTGCACTCGGAGAA	60 °C
AKT1	GTGCCGCAAAAGGTCTTCATGG	60 °C
SDF1	CTCAACACTCCAAACTGTGCCC	60 °C
SDF1	CTCCAGGTACTCCTGAATCCAC	60 °C
SIRT1	TAGACACGCTGGAACAGGTTGC	60 °C
SIRT1	CTCCTCGTACAGCTTCACAGTC	60 °C
IGF1	CTCTTCAGTTCGTGTGTGGAGAC	60 °C
IGF1	CAGCCTCCTTAGATCACAGCTC	60 °C
P16	CTCGTGCTGATGCTACTGAGGA	60 °C
P16	GGTCGGCGCAGTTGGGCTCC	60 °C
P21	AGGTGGACCTGGAGACTCTCAG	60 °C
P21	TCCTCTTGGAGAAGATCAGCCG	60 °C
P53	CCTCAGCATCTTATCCGAGTGG	60 °C
P53	TGGATGGTGGTACAGTCAGAGC	60 °C
BAX	TCAGGATGCGTCCACCAAGAAG	60 °C
BAX	TGTGTCCACGGCGGCAATCATC	60 °C
BAK	TTACCGCCATCAGCAGGAACAG	60 °C
BAK	GGAACTCTGAGTCATAGCGTCG	60 °C

VEGF: Vascular endothelial growth factor; AKT1: Protein kinase B; SDF1: Stromal cell-derived factor-1; SIRT1: Sirtuin 1; IGF1: Insulin-like growth factor 1.

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Citation: Bashir B, Choudhery MS, Hussain I. Hypoxic preconditioning restores the regenerative potential of aged adipose tissue derived mesenchymal stem cells. World J Stem Cells 2026; 18(5): 117241
URL: https://www.wjgnet.com/1948-0210/full/v18/i5/117241.htm
DOI: https://dx.doi.org/10.4252/wjsc.v18.i5.117241

Bashir B, Choudhery MS, Hussain I. Hypoxic preconditioning restores the regenerative potential of aged adipose tissue derived mesenchymal stem cells. World J Stem Cells 2026; 18(5): 117241 [DOI: 10.4252/wjsc.v18.i5.117241]

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