RNA interference-mediated osteoprotegerin silencing increases the receptor activator of nuclear factor-kappa B ligand/osteoprotegerin ratio and promotes osteoclastogenesis

Table 1 Short hairpin RNA nucleotide sequences used for RNA interference

Name	shRNA primer sequence (5’-3’)
shOPG (F)	5’-CCGGACTTGCATTATGACCCAGAACTCGAGTTTCTGGGTCATAATGCAAGTTTTTTG-3’
shOPG (R)	5’-AATTCAAAAAACTTGCATTATGACCCAGAAACTCGAGTTTCTGGGTCATAATGCAAGT-3’

shRNA: Short hairpin RNA; OPG: Osteoprotegerin; F: Forward; R: Reverse.

Table 2 Nucleotide sequences of the primers used for real-time quantitative polymerase chain reaction

Gene	Primer sequence (5’-3’)	PCR product (bp)
RANKL	5’-TTACCTGTACGCCAACATTTGC-3’ (F)	283
	5’-AAGTACGTCGCATCTTGATCC-3’ (R)
OPG	5’-TGTTCTGGTGGACAGTTTGC-3’ (F)	167
	5’-AGAGGTCAATGTCTTGGAT-3’ (R)
CTSK	5’-ATATGTGCAGCAGAATGGAGG-3’ (F)	195
	5’-CTTGCATCGATGGACACAGAG-3’ (R)
TRAP	5’-CCACAACCTGCAGTATCTTC-3’ (F)	200
	5’-CCACATACGTGATGCTCATTTC-3’ (R)
MMP-9	5’-TTGCCTGCAAAGTTGAACTCAG-3’ (F)	264
	5’-CAAGCGAGTAACGCTCTGG-3’ (R)
CTR	5’-AGCTGGTGTAATGTCCTATCAG-3’ (F)	236
	5’-CAATCAGAGCAGCAATTGACATGG-3’ (R)
β-actin	5’-GGAGATTACTGCCCTGGCTCCTA-3’ (F)	150
	5’-GACTCATCGTACTCCTGCTTGCTG-3’ (R)

PCR: Polymerase chain reaction; RANKL: Receptor activator of nuclear factor-kappa B ligand; OPG: Osteoprotegerin; CTSK: Cathepsin K; TRAP: Tartrate-resistant acid phosphatase; MMP-9: Matrix metalloproteinase-9; CTR: Receptors for calcitonin; F: Forward; R: Reverse.