Transcription regulators differentiate mesenchymal stem cells into chondroprogenitors, and their in vivo implantation regenerated the intervertebral disc degeneration

Table 1 Primer sequences used in the study with their annealing temperatures

Gene	Primer sequences (5’-3’)	Annealing temperature (ºC)
GAPDH	(F) 5’-CACCATGGGGAAGGTGAAGG-3’; (R) 5’- AGCATCGCCCCACTTGATTT -3’	58
β-actin	(F) 5’-CACTGGCATCGTGATGGACT -3’; (R) 5’-TGGCCATCTCTTGCTCGAAG -3’	58
Sox-9	(F) 5’-CATCTCCCCCAACGCCA-3’; (R) 5’-TGGGATTGCCCCGAGTG-3’	58
Six-1	(F) 5’-CTCCAGTCTGGTGGACTTGG-3’; (R) 5’-AGCTTGAGATCGCTGTTGGT -3’	58
BMP2	(F) 5’-AGCTGGGCCGCAGGA -3’; (R) 5’-TCGGCTGGCTGCCCT -3’	58
Aggrecan	(F) 5’-AATCTCACAATGCCACGCTG -3’; (R) 5’-GAGGCTGCATACCTCGGAAG -3’	58
TGFβ1	(F) 5’-CAAGGCACAGGGGACCAG -3’; (R) 5’-CAGGTTCCTGGTGGGCAG -3’	58