Spinal cord injury regeneration using autologous bone marrow-derived neurocytes and rat embryonic stem cells: A comparative study in rats

Table 1 Basso, Beattie, Brenham scale following treatment with control, rat embryonic stem cells and autologous bone marrow-derived neurocytes

Week	Control	rESC	ABNDN
1	0	0	0
2	0	0	3
3	0	3	3
4	1	3	4
5	1	5	6
6	1	7	8
7	2	7	10
8	2	11	13

rESC: Rat embryonic stem cells; ABMDN: Autologous bone marrow-derived neurocytes.

Table 2 Train-of-four-Watch record for the control group

	Mode	Tw1 (%)	Tw2 (%)	Tw3 (%)	Tw4 (%)	TOF (%)	Temp (°C)	Stim (mA)	T (μs)	Sens.
1	TOF	4	0	0	1	25	33.6	50	200	157
2	TOF	0	0	0	0		33.1	50	200	157
3	TOF	0	0	0	0		32.6	50	200	157
4	TOF	0	0	0	0		33.3	50	200	157
5	TOF	0	0	0	0		32.7	50	200	157
6	TOF	0	0	0	0		327	50	200	157
7	TOF	6	3	3	2	33.3	32.9	50	200	157
8	TOF	4	3	0	3	52	33.8	50	200	157
9	TOF	0	0	0	0		32.4	50	200	157
10	TOF	0	0	0	0		32.0	50	200	157
11	TOF	0	0	0	0		32.7	50	200	157
12	TOF	5	0	2	3	60	33.1	50	200	157
13	TOF	4	3	3	0		32.9	50	200	157
14	TOF	0	0	0	0		33.1	50	200	157
15	TOF	7	3	4	3	42	33.6	50	200	157

TOF: Train-of-four.

Table 3 Train-of-four-Watch record for the rat embryonic stem cells group

	Mode	Tw1 (%)	Tw2 (%)	Tw3 (%)	Tw4 (%)	TOF (%)	Temp (°C)	Stim (mA)	T (μs)	Sens.
1	TOF	85	81	67	86	96	32.8	50	200	157
2	TOF	87	43	56	60	86	33.1	50	200	157
3	TOF	76	57	52	42	68	33.2	50	200	157
4	TOF	79	71	78	65	82	32.8	50	200	157
5	TOF	65	77	43	59	46	33.1	50	200	157
6	TOF	52	57	52	42	80	32.2	50	200	157
7	TOF	43	77	75	75	89	31.8	50	200	157
8	TOF	33	34	34	30	90	31.1	50	200	157
9	TOF	52	57	52	42	81	31.2	50	200	157
10	TOF	66	48	69	24	88	32.8	50	200	157
11	TOF	33	34	34	65	90	33.1	50	200	157
12	TOF	52	57	52	42	80	33.2	50	200	157
13	TOF	75	71	72	24	88	32.8	50	200	157
14	TOF	39	34	55	70	76	32.1	50	200	157
15	TOF	56	57	52	42	75	33.6	50	200	157

(Twitch 4/Twitch 1) × 100 (%). TOF: Train-of-four.

Table 4 Train-of-four-Watch record for the autologous bone marrow-derived neurocytes group

	Mode	Tw1 (%)	Tw2 (%)	Tw3 (%)	Tw4 (%)	TOF (%)	Temp (°C)	Stim (mA)	T (μs)	Sens.
1	TOF	45	61	66	44	67	32.1	50	200	157
2	TOF	39	24	34	28	71	32.5	50	200	157
3	TOF	58	53	42	32	55	33.2	50	200	157
4	TOF	62	61	87	45	72	32.8	50	200	157
5	TOF	49	67	35	34	57	32.9	50	200	157
6	TOF	60	59	51	50	69	32.7	50	200	157
7	TOF	61	23	29	43	70	33.2	50	200	157
8	TOF	66	43	61	39	59	32.6	50	200	157
9	TOF	52	49	51	44	84	31.9	50	200	157
10	TOF	67	31	27	37	55	32.3	50	200	157
11	TOF	39	43	54	29	74	32.8	50	200	157
12	TOF	65	64	57	35	45	32.7	50	200	157
13	TOF	58	61	59	61	67	32.9	50	200	157
14	TOF	53	60	51	49	77	32.1	50	200	157
15	TOF	62	59	56	61	79	32.6	50	200	157

(Twitch 4/Twitch 1) × 100 (%). TOF: Train-of-four.

Table 5 Comparison of electromyographic recording in the three groups

Variable	Control	rESC	ABMDN
Twitch1 height	2	49.5	52.9
Twitch2 height	0.8	50	48.7
Twitch3 height	0.8	51	43.7
Twitch4 height	0.8	42	38.9
TOF ratio = (Twitch 4/Twitch 1) × 100 (%)	47.6 ± 20.3	69.33 ± 13.9	80.2 ± 11.9
Stimulation current (60 mA)	50	50	50
Pulse width (200 μs)	200	200	200

rESC: Rat embryonic stem cells; ABMDN: Autologous bone marrow-derived neurocytes; TOF: Train-of-four.