Basic Study
Copyright ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Dec 26, 2020; 12(12): 1591-1602
Published online Dec 26, 2020. doi: 10.4252/wjsc.v12.i12.1591
Spinal cord injury regeneration using autologous bone marrow-derived neurocytes and rat embryonic stem cells: A comparative study in rats
Mir Sadat-Ali, Dakheel A Al-Dakheel, Ayesha Ahmed, Haifa A Al-Turki, Abdallah S Al-Omran, Sadananda Acharya, Methal I Al-Bayat
Mir Sadat-Ali, Department of Orthopedic Surgery, Imam Abdulrahman Bin Faisal University and King Fahd Hospital of the University, Dammam 31952, Saudi Arabia
Dakheel A Al-Dakheel, Department of Orthopedic Surgery, Imam Abdulrahman Bin Faisal University, AlKhobar 31952, Saudi Arabia
Ayesha Ahmed, Methal I Al-Bayat, Department of Pathology, Imam Abdulrahman Bin Faisal University, Dammam 31952, Saudi Arabia
Haifa A Al-Turki, Department of Obstetrics and Gynecology, Imam Abdulrahman Bin Faisal University, Dammam 31142, Saudi Arabia
Abdallah S Al-Omran, Department of Orthopedic Surgery, Imam Abdulrahman Bin Faisal University and King Fahd Hospital of the University, AlKhobar 31952, Saudi Arabia
Sadananda Acharya, Department of Public Health, College of Public Health, Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia
Author contributions: All authors participated equally from the inception to operative work, culturing neurocytes and histology.
Supported by King Abdulaziz City for Science and Technology, Riyadh vide, No. 10-MED1091-46.
Institutional animal care and use committee statement: The Committee of the Animal Care of the IAU gave the approval and monitored the study.
Conflict-of-interest statement: All authors have no conflicts of interest related to the manuscript.
Data sharing statement: The data are available for review at the dsr@iau.edu.sa.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Mir Sadat-Ali, BM BCh, FRCS (Gen Surg), MBBS, MSc, PhD, Full Professor, Doctor, Department of Orthopedic Surgery, Imam Abdulrahman Bin Faisal University and King Fahd Hospital of the University, PO Box 40071, Dammam 31952, Saudi Arabia. drsadat@hotmail.com
Received: March 21, 2020
Peer-review started: March 21, 2020
First decision: August 22, 2020
Revised: August 24, 2020
Accepted: November 12, 2020
Article in press: November 12, 2020
Published online: December 26, 2020
Processing time: 280 Days and 11.9 Hours
ARTICLE HIGHLIGHTS
Research background

Different methods have been used to heal damaged neural tissue and the results have not been promising.

Research motivation

Many young people have been paralyzed due to trauma and adequate treatment to heal neural tissue is unavailable.

Research objectives

To heal damaged neural tissue using stem cells.

Research methods

Wistar rats were paralyzed using direct trauma and then treated with rat embryonic stem cells (rESC), autologous bone marrow-derived neurocytes (ABMDN) and saline and the results were observed using electromyography and histology.

Research results

ABMDN resulted in significantly better recovery than rESC.

Research conclusions

ABMDN led to significantly better recovery than rESC in rats with iatrogenic SCI.

Research perspectives

These findings provide adequate evidence for conducting trials in larger animals followed by phase I human trials.