Takahashi Y, Tomotsune D, Takizawa S, Yue F, Nagai M, Yokoyama T, Hirashima K, Sasaki K. New model for cardiomyocyte sheet transplantation using a virus-cell fusion technique. World J Stem Cells 2015; 7(5): 883-893 [PMID: 26131319 DOI: 10.4252/wjsc.v7.i5.883]
Corresponding Author of This Article
Katsunori Sasaki, MD, Department of Histology and Embryology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan. katsmd@shinshu-u.ac.jp
Research Domain of This Article
Transplantation
Article-Type of This Article
Basic Study
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Jun 26, 2015 (publication date) through Feb 17, 2026
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Publication Name
World Journal of Stem Cells
ISSN
1948-0210
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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
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Takahashi Y, Tomotsune D, Takizawa S, Yue F, Nagai M, Yokoyama T, Hirashima K, Sasaki K. New model for cardiomyocyte sheet transplantation using a virus-cell fusion technique. World J Stem Cells 2015; 7(5): 883-893 [PMID: 26131319 DOI: 10.4252/wjsc.v7.i5.883]
World J Stem Cells. Jun 26, 2015; 7(5): 883-893 Published online Jun 26, 2015. doi: 10.4252/wjsc.v7.i5.883
New model for cardiomyocyte sheet transplantation using a virus-cell fusion technique
Yuto Takahashi, Daihachiro Tomotsune, Sakiko Takizawa, Fengming Yue, Mika Nagai, Tadayuki Yokoyama, Kanji Hirashima, Katsunori Sasaki
Yuto Takahashi, Daihachiro Tomotsune, Fengming Yue, Kanji Hirashima, Katsunori Sasaki, Department of Histology and Embryology, Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan
Daihachiro Tomotsune, Katsunori Sasaki, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Matsumoto, Nagano 390-8621, Japan
Sakiko Takizawa, Mika Nagai, Tadayuki Yokoyama, Bourbon Corporation, Kashiwazaki, Niigata 945-8611, Japan
Author contributions: Takahashi Y designed and performed the majority of the experiments, interpreted the data, and revised the article; Tomotsune D and Yue F helped to design the experiments, obtain the data, and interpret the results; Takizawa S, Nagai M and Yokoyama T provided technical assistance in obtaining the data; Hirashima K was associated with completing the article; Sasaki K helped to design the experiments and wrote the article.
Supported by A Grant-in-Aid for Scientific Research from the Japanese Ministry of Education, Science and Sports, No. 24240076.
Ethics approval: The study was reviewed and approved by the Animal Care Committee of Shinshu University, which belongs to an institution of the university as well as the Animal Center of Shinshu University and referees a research plan on the view of ethics, science and animal welfare.
Institutional animal care and use committee: All procedures were reviewed and approved by the Institutional Animal Care and Use Committee of the Animal Center of Shinshu University. It is the same institution as The Animal Care Committee of Sinshu University (Approved protocol number: 210018; 240025).
Conflict-of-interest: All authors have declared that no conflicts of interest exist.
Data sharing: No additional data are available.
Correspondence to: Katsunori Sasaki, MD, Department of Histology and Embryology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan. katsmd@shinshu-u.ac.jp
Telephone: +81-263-372589 Fax: +81-263-373093
Received: October 17, 2014 Peer-review started: October 20, 2014 First decision: December 17, 2014 Revised: March 6, 2015 Accepted: April 1, 2015 Article in press: April 7, 2015 Published online: June 26, 2015 Processing time: 255 Days and 17.7 Hours
Core Tip
Core tip: It is very important to produce close contacts between transplanted cell sheets and host cells without fibrous invasion for tissue reconstruction and function. Unfortunately, cellular transplantation research has not focused on this problem and convenient methods have not yet been developed. In this study using hemagglutinating virus of Japan envelope, transplanted cardiomyocyte sheets were placed in direct contact with host skeletal muscles. By adding NaOH maceration of connective tissues, rich, largely-movable, beating domains were observed. Histological observations revealed successful formation of close contacts between the two cell types. This paper proposes a new idea of introducing artificial cell fusion into cellular transplantation methods.
