©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Dec 26, 2015; 7(11): 1233-1250
Published online Dec 26, 2015. doi: 10.4252/wjsc.v7.i11.1233
Published online Dec 26, 2015. doi: 10.4252/wjsc.v7.i11.1233
Towards in vivo amplification: Overcoming hurdles in the use of hematopoietic stem cells in transplantation and gene therapy
Murtaza S Nagree, Department of Medical Biophysics, Faculty of Medicine, University of Toronto, Toronto, ON M5G 1L7, Canada
Lucía López-Vásquez, Institute of Medical Science, Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada
Jeffrey A Medin, University Health Network, Toronto, ON M5G 1L7, Canada
Author contributions: Nagree MS, López-Vásquez L and Medin JA wrote the manuscript.
Supported by Canadian Institutes of Health Research Grant to Medin JA, No. MOP-123528.
Conflict-of-interest statement: Authors declare no conflict of interests for this article.
Correspondence to: Jeffrey A Medin, PhD, University Health Network, 5R406 Max Bell Research Centre, 101 College Street, Toronto, ON M5G 1L7, Canada. jmedin@uhnresearch.ca
Telephone: +1-416-3404745
Received: August 8, 2015
Peer-review started: August 8, 2015
First decision: September 22, 2015
Revised: October 8, 2015
Accepted: November 23, 2015
Article in press: November 25, 2015
Published online: December 26, 2015
Processing time: 139 Days and 3.3 Hours
Peer-review started: August 8, 2015
First decision: September 22, 2015
Revised: October 8, 2015
Accepted: November 23, 2015
Article in press: November 25, 2015
Published online: December 26, 2015
Processing time: 139 Days and 3.3 Hours
Core Tip
Core tip: Though hematopoietic stem cell (HSC)-directed gene therapy is becoming a viable therapy for many disorders, optimization of clinical output needs improvement. One approach to circumvent lower efficiencies of gene transfer and/or engraftment is to apply in vivo amplification strategies. Here we review various modules that have been developed and tested to mediate amplification of HSCs after gene transfer.