©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Jan 26, 2015; 7(1): 116-125
Published online Jan 26, 2015. doi: 10.4252/wjsc.v7.i1.116
Published online Jan 26, 2015. doi: 10.4252/wjsc.v7.i1.116
Methods of induced pluripotent stem cells for clinical application
Tomohisa Seki, Keiichi Fukuda, Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan
Tomohisa Seki, Department of Emergency and Critical Care Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan
Author contributions: Seki T prepared most of the paper; Fukuda K provided advice and proofread the paper.
Supported by The Support Program to break the bottlenecks at R&D Systems for accelerating the practical use of Health Research Outcome, the Highway Program for the Realization of Regenerative Medicine, and Health Labour Sciences Research Grant.
Correspondence to: Keiichi Fukuda, MD, PhD, FACC, Professor and Chair, Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. kfukuda@a2.keio.jp
Telephone: +81-3-53633874 Fax: +81-3-53633875
Received: July 27, 2014
Peer-review started: July 28, 2014
First decision: September 4, 2014
Revised: September 18, 2014
Accepted: October 23, 2014
Article in press: December 16, 2014
Published online: January 26, 2015
Processing time: 170 Days and 14.9 Hours
Peer-review started: July 28, 2014
First decision: September 4, 2014
Revised: September 18, 2014
Accepted: October 23, 2014
Article in press: December 16, 2014
Published online: January 26, 2015
Processing time: 170 Days and 14.9 Hours
Core Tip
Core tip: Each induced pluripotent stem cells methodology has advantages and disadvantages, as in the case of autologous vs allogenic transplantation, and the choice of appropriate strategy may vary depending on the intended use. Additionally, to avoid tumorigenesis and to establish effective differentiation into the intended cells, further investigation is needed to identify the most suitable iPSC line and how these lines should be selected.